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Physico-chemical foundations underpinning microarray and next-generation sequencing experiments
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Harrison, Andrew B., Binder, Hans, Buhot, Arnaud , Burden, Conrad John, Carlon, Enrico, Gibas, Cynthia, Gamble, Lara J., Halperin, Avraham, Hooyberghs, Jef, Kreil, David, Levicky, Rastislav, Noble, Peter A., Ott, Albrecht, Pettitt, B. Montgomery, Tautz, Diethard and Pozhitkov, Alexander E. (2013) Physico-chemical foundations underpinning microarray and next-generation sequencing experiments. Nucleic Acids Research, Volume 41 (Number 5). pp. 2779-2796. doi:10.1093/nar/gks1358 ISSN 0305-1048.
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Official URL: http://dx.doi.org/10.1093/nar/gks1358
Abstract
Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS). A physical understanding of the hybridization process helps to determine the accuracy of these technologies. The goal of a widespread research program is to develop reliable transformations between the raw signals reported by the technologies and individual molecular concentrations from an ensemble of nucleic acids. This research has inputs from many areas, from bioinformatics and biostatistics, to theoretical and experimental biochemistry and biophysics, to computer simulations. A group of leading researchers met in Ploen Germany in 2011 to discuss present knowledge and limitations of our physico-chemical understanding of high-throughput nucleic acid technologies. This meeting inspired us to write this summary, which provides an overview of the state-of-the-art approaches based on physico-chemical foundation to modeling of the nucleic acids hybridization process on solid surfaces. In addition, practical application of current knowledge is emphasized.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QA Mathematics Q Science > QC Physics Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | DNA microarrays, Genes, Biomolecules, Nucleic acids | ||||
Journal or Publication Title: | Nucleic Acids Research | ||||
Publisher: | Oxford University Press | ||||
ISSN: | 0305-1048 | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | Volume 41 | ||||
Number: | Number 5 | ||||
Page Range: | pp. 2779-2796 | ||||
DOI: | 10.1093/nar/gks1358 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 24 December 2015 | ||||
Date of first compliant Open Access: | 24 December 2015 | ||||
Funder: | Deutsche Akademie der Naturforscher Leopoldina, Max-Planck-Gesellschaft zur Förderung der Wissenschaften [Max Planck Society for the Advancement of Science], Agilent Technologies, National Institutes of Health (U.S.) (NIH), National Science Foundation (U.S.) (NSF), Wiener Wissenschafts, Forschungs und Technologiefonds [Vienna Science and Technology Fund] (WWTF), Baxter International Inc., Österreichisches Forschungszentrum Seibersdorf [Austrian Research Centre Seibersdorf] (ARCS), Austrian Center of Biopharmaceutical Technology (ACBT) | ||||
Grant number: | EB-001473 (NIH), NESAC/BIO (NIH), EB-002027 (NIH), DBI 10388671 (NSF), CREST HRD 1241701 (NSF), DMR-1206754 (NSF) |
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