The Library
Topoisomerase IIα promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation
Tools
Ray, Swagat, Panova, Tatiana, Miller, G. (Gail), Volkov, Arsen, Porter, Andrew C. G., Russell, J. (Jackie), Panov, Konstantin I. and Zomerdijk, Joost C. B. M. (2013) Topoisomerase IIα promotes activation of RNA polymerase I transcription by facilitating pre-initiation complex formation. Nature Communications, Vol.4 . Article: 1598. doi:10.1038/ncomms2599 ISSN 2041-1723.
|
Text
WRAP_Ray_ncomms2599.pdf - Published Version Available under License Creative Commons Attribution Non-commercial Share Alike. Download (2587Kb) | Preview |
Official URL: http://dx.doi.org/10.1038/ncomms2599
Abstract
Type II DNA topoisomerases catalyse DNA double-strand cleavage, passage and re-ligation to effect topological changes. There is considerable interest in elucidating topoisomerase II roles, particularly as these proteins are targets for anti-cancer drugs. Here we uncover a role for topoisomerase IIα in RNA polymerase I-directed ribosomal RNA gene transcription, which drives cell growth and proliferation and is upregulated in cancer cells. Our data suggest that topoisomerase IIα is a component of the initiation-competent RNA polymerase Iβ complex and interacts directly with RNA polymerase I-associated transcription factor RRN3, which targets the polymerase to promoter-bound SL1 in pre-initiation complex formation. In cells, activation of rDNA transcription is reduced by inhibition or depletion of topoisomerase II, and this is accompanied by reduced transient double-strand DNA cleavage in the rDNA-promoter region and reduced pre-initiation complex formation. We propose that topoisomerase IIα functions in RNA polymerase I transcription to produce topological changes at the rDNA promoter that facilitate efficient de novo pre-initiation complex formation.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QP Physiology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | DNA topoisomerase II, RNA polymerases, DNA topoisomerase II -- Therapeutic use, Antineoplastic agents, RNA-protein interactions, Genetic transcription | ||||
Journal or Publication Title: | Nature Communications | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 2041-1723 | ||||
Official Date: | 19 March 2013 | ||||
Dates: |
|
||||
Volume: | Vol.4 | ||||
Page Range: | Article: 1598 | ||||
DOI: | 10.1038/ncomms2599 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 24 December 2015 | ||||
Date of first compliant Open Access: | 24 December 2015 | ||||
Funder: | Cancer Research UK (CRUK), Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England) | ||||
Grant number: | C9700/A5962 (CRUK), 89365 (MRC), 085441/Z/08/Z (WT) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year