Malfolded recombinant tat substrates are tat-independently degraded in Escherichia coli
Lindenstrauss, Ute, Matos, Cristina F. R. O., Graubner, Wenke, Robinson, Colin and Brueser, Thomas. (2010) Malfolded recombinant tat substrates are tat-independently degraded in Escherichia coli. FEBS Letters, Vol.584 (No.16). pp. 3644-3648. ISSN 0014-5793Full text not available from this repository.
Official URL: http://dx.doi.org/10.1016/j.febslet.2010.07.039
The twin-arginine translocation (Tat) system translocates folded proteins across biological membranes. It has been suggested that the Tat system of Escherichia coli can direct Tat substrates to degradation if they are not properly folded [Matos, C. F., Robinson, C. and Di Cola, A. (2008) The Tat system proofreads FeS protein substrates and directly initiates the disposal of rejected molecules. EMBO J. 27, 2055-2063; Matos, C. F., Di Cola, A. and Robinson, C. (2009) TatD is a central component of a Tat translocon-initiated quality control system for exported FeS proteins in Escherichia coli. EMBO Rep. 10, 474-479]. Contrary to the earlier reports, it is now concluded that reported differences between tested strains were due to variations in expression levels and inclusion body formation. Using the native Tat substrate NrfC and a malfolded variant thereof, we show that the turnover of these proteins is not affected by the absence of all known Tat components. Malfolded NrfC is degraded more quickly than the native protein, indicating that Tat-independent protease systems can recognize malfolded Tat substrates. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
|Item Type:||Journal Article|
|Subjects:||Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||FEBS Letters|
|Official Date:||20 August 2010|
|Number of Pages:||5|
|Page Range:||pp. 3644-3648|
|Access rights to Published version:||Restricted or Subscription Access|
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