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Fluoroquinolone resistance in Campylobacter species from man and animals : detection of mutations in topoisomerase genes
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Piddock, Laura J. V., Ricci, Vito , Pumbwe, Lilian , Everett , Martin J. and Griggs, Deborah (2003) Fluoroquinolone resistance in Campylobacter species from man and animals : detection of mutations in topoisomerase genes. Journal of Antimicrobial Chemotherapy, Vol.51 (No.1). pp. 19-26. doi:10.1093/jac/dkg033 ISSN 0305-7453.
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Official URL: http://dx.doi.org/10.1093/jac/dkg033
Abstract
Consecutive isolates of quinolone-resistant campylobacter isolated over a 5 year period (1990–1995) from the faeces of patients with enteritis in Plymouth, UK, were examined for the epidemiology of mutations in gyrA (n = 127). In addition, clinical isolates and poultry isolates from Germany, The Netherlands and other regions of the UK collected before 1995 were examined for mutations in the quinolone resistance-determining region of gyrA by single-stranded conformational polymorphism analysis and direct sequencing of a 270 bp fragment of PCR-generated DNA. The majority of isolates (173/208) carried a mutation at codon 86 in gyrA resulting in substitution of Ile for Thr; all of these were resistant to ciprofloxacin (MIC ≥ 2 mg/L). One isolate of Campylobacter jejuni had a mutation at Asp-90, and another had a double mutation at Thr-86 and Pro-104. Only two resistant isolates showed no mutation in gyrA. A novel gyrA sequence was amplified from two Campylobacter lari and one C. jejuni, which exhibited a valine at codon 86. Only 8/192 isolates had changes in gyrB; all were shown to relate to silent mutations in gyrB and presumably reflect natural polymorphisms in the gene.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
| Journal or Publication Title: | Journal of Antimicrobial Chemotherapy | ||||
| Publisher: | Oxford University Press | ||||
| ISSN: | 0305-7453 | ||||
| Official Date: | 2003 | ||||
| Dates: |
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| Volume: | Vol.51 | ||||
| Number: | No.1 | ||||
| Page Range: | pp. 19-26 | ||||
| DOI: | 10.1093/jac/dkg033 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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