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Evidence for an efflux pump mediating multiple antibiotic resistance in Salmonella enterica serovar Typhimurium

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Piddock, Laura J. V., White, D. G., Gensberg, K., Pumbwe, L. and Griggs, Deborah (2000) Evidence for an efflux pump mediating multiple antibiotic resistance in Salmonella enterica serovar Typhimurium. Antimicrobial Agents and Chemotherapy, Vol.44 (No.11). pp. 3118-3121. doi:10.1128/AAC.44.11.3118-3121.2000 ISSN 0066-4804.

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Official URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC101613...

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Abstract

The mechanism of multiple antibiotic resistance in six isolates of Salmonella enterica serovar Typhimurium recovered from a patient treated with ciprofloxacin was studied to investigate the role of efflux in the resistance phenotype. Compared to the patient's pretherapy isolate (L3), five of six isolates accumulated less ciprofloxacin, three of six isolates accumulated less chloramphenicol, and all six accumulated less tetracycline. The accumulation of one or more antibiotics was increased by carbonyl cyanide m-chlorophenylhydrazone to concentrations similar to those accumulated by L3 for all isolates except one, in which accumulation of all three agents remained approximately half that of L3. All isolates had the published wild-type sequences of marO and marR. No increased expression of marA, tolC, or soxS was observed by Northern blotting; however, three isolates showed increased expression of acrB, which was confirmed by quantitative competitive reverse transcription-PCR. However, there were no mutations within acrR or the promoter region of acrAB in any of the isolates.

Item Type: Journal Article
Divisions: Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School
Journal or Publication Title: Antimicrobial Agents and Chemotherapy
Publisher: American Society for Microbiology
ISSN: 0066-4804
Official Date: November 2000
Dates:
DateEvent
November 2000Published
Volume: Vol.44
Number: No.11
Page Range: pp. 3118-3121
DOI: 10.1128/AAC.44.11.3118-3121.2000
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access

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