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Selection and characterization of cefepime-resistant Gram-negative bacteria
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Piddock, Laura J. V. and Griggs, Deborah (1991) Selection and characterization of cefepime-resistant Gram-negative bacteria. Journal of Antimicrobial Chemotherapy, Vol.28 (No.5). pp. 669-676. doi:10.1093/jac/28.5.669 ISSN 0305-7453.
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Official URL: http://dx.doi.org/10.1093/jac/28.5.669
Abstract
The NCTC type strains and four clinical isolates of Enterobacter cloacae, Citrobacter freundii, Serratia marcescens, Morganella morganii, Providencia stuartii and Pseudomonas aeruginosa were exposed, in agar, to cefepime at 3, 5, and 10 × MIC and a breakpoint concentration of 16 mg/L. Mutants were selected at a frequency of ˜ 10−2 that had decreased susceptibility to cefepime and cefpirome, and species-dependent resistance to other β-lactams. Any putative mutant with a greater than four-fold increase in the MIC was examined to determine its β-lactamase expression and outer membrane protein (Omp) profile. Mutant strains of P. stuartii and M. morganii lacked an Omp of molecular mass similar to that of OmpF, and were cross-resistant to nalidixic acid. Mutant strains of E. cloacae had derepressed class I β-lactamase production and lacked an Omp corresponding to OmpF, suggesting that in this species both parameters are necessary for decreased susceptibility. Derepressed β-lactamases purified from mutant strains of E. cloacae, C. freundii and P. stuartii was able to hydrolyse cefepime, but not as quickly as TEM-10.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
Journal or Publication Title: | Journal of Antimicrobial Chemotherapy | ||||
Publisher: | Oxford University Press | ||||
ISSN: | 0305-7453 | ||||
Official Date: | 1991 | ||||
Dates: |
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Volume: | Vol.28 | ||||
Number: | No.5 | ||||
Page Range: | pp. 669-676 | ||||
DOI: | 10.1093/jac/28.5.669 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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