Dowsett, M., Mansfield, M. D., Griggs, Deborah and Jeffcoate, S. L. (1984) Validation and use of centrifugal ultrafiltration-dialysis in the measurement of percent free oestradiol in serum. Journal of Steroid Biochemistry, Vol.21 (No.3). pp. 343-345. doi:10.1016/0022-4731(84)90290-5

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Abstract

The validity of the centrifugal ultrafiltration-dialysis method in the measurement of the percentage of oestradiol and testosterone which is not bound to protein (free) in human serum was assessed. In an initial validation, the values of % free steroid obtained for oestradiol and testosterone in protein-free ultrafiltrates of human serum were higher than expected. Filter paper discs used in the system were shown to adsorb [3H]oestradiol from protein-free solution. Their replacement with glass-fibre discs, which did not adsorb oestradiol, decreased but did not eliminate the discrepancy between observed and expected results for % free oestradiol in ultrafiltrates. The use of polyethylene rather than polypropylene vials then reduced the discrepancy to insignificant levels. The estimates for % free oestradiol in serum were reduced by these modifications.

Item Type:	Journal Article
Divisions:	Faculty of Medicine > Warwick Medical School
Journal or Publication Title:	Journal of Steroid Biochemistry
Publisher:	Pergamon
ISSN:	0022-4731
Official Date:	1984
Dates:	Date Event 1984 Published
Volume:	Vol.21
Number:	No.3
Page Range:	pp. 343-345
DOI:	10.1016/0022-4731(84)90290-5
Status:	Peer Reviewed
Access rights to Published version:	Restricted or Subscription Access

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