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Organization of the LEE1 operon regulatory region of enterohaemorrhagic Escherichia coli O157:H7 and activation by GrlA
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Islam, Md. Shahidul, Bingle, Lewis E. H., Pallen, Mark J. and Busby, Stephen J. W. (2010) Organization of the LEE1 operon regulatory region of enterohaemorrhagic Escherichia coli O157:H7 and activation by GrlA. Molecular Microbiology, 79 (2). pp. 468-483. doi:10.1111/j.1365-2958.2010.07460.x ISSN 0950-382X.
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Official URL: http://dx.doi.org/10.1111/j.1365-2958.2010.07460.x
Abstract
Expression of the genes in the locus of enterocyte effacement (LEE) in enterohaemorrhagic Escherichia coli is primarily co-ordinated by expression of the LEE1 operon. GrlA is a LEE-encoded transcription regulator that has been proposed to be involved in the regulation of expression of the LEE1 operon. We describe a simple plasmid-based system to investigate the LEE1 operon regulatory region and to study GrlA-dependent effects. We report that GrlA can activate transcription initiation at the LEE1 P1 promoter by binding to a target located within the 18-base-pair spacer between the promoter −10 and −35 elements, which were defined by mutational analysis. Shortening this spacer to 17 base pairs increases P1 promoter activity and short-circuits GrlA-dependent activation. Hence, at the P1 promoter, the action of GrlA resembles that of many MerR family transcription activators at their target promoters.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Molecular Microbiology | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 0950-382X | ||||
Official Date: | 2010 | ||||
Dates: |
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Volume: | 79 | ||||
Number: | 2 | ||||
Page Range: | pp. 468-483 | ||||
DOI: | 10.1111/j.1365-2958.2010.07460.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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