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Functional analysis of interaction sites on the n-terminal domain of clathrin heavy chain
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Willox, Anna K. and Royle, Stephen J. (2012) Functional analysis of interaction sites on the n-terminal domain of clathrin heavy chain. Traffic, 13 (1). pp. 70-81. doi:10.1111/j.1600-0854.2011.01289.x ISSN 1398-9219.
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Official URL: http://dx.doi.org/10.1111/j.1600-0854.2011.01289.x
Abstract
In clathrin-mediated membrane traffic, clathrin does not bind directly to cargo and instead binds to adaptors that mediate this function. For endocytosis, the main adaptor is the adaptor protein (AP)-2 complex, but it is uncertain how clathrin contacts AP-2. Here we tested in human cells the importance of the three binding sites that have been identified so far on the N-terminal domain (NTD) of clathrin. We find that mutation of each of the three sites on the NTD, alone or in combination, does not block clathrin/AP-2-mediated endocytosis in the same way as deletion of the NTD. We report here the fourth and final site on the NTD that is required for clathrin/AP-2-mediated endocytic function. Each of the four interaction sites can operate alone to mediate endocytosis. The observed functional redundancy between interaction sites on the NTD explains how productivity of clathrin-coated vesicle formation is ensured.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School | ||||
Journal or Publication Title: | Traffic | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 1398-9219 | ||||
Official Date: | January 2012 | ||||
Dates: |
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Volume: | 13 | ||||
Number: | 1 | ||||
Page Range: | pp. 70-81 | ||||
DOI: | 10.1111/j.1600-0854.2011.01289.x | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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