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miR-132/212 knockout mice reveal roles for these miRNAs in regulating cortical synaptic transmission and plasticity
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Remenyi, Judit, van den Bosch, Mirjam W. M., Palygin, Oleg, Mistry, Rajen B., McKenzie, Colin, Macdonald, Andrew, Hutvagner, Gyorgy, Arthur, J. Simon C., Frenguelli, Bruno G. and Pankratov, Yuriy (2013) miR-132/212 knockout mice reveal roles for these miRNAs in regulating cortical synaptic transmission and plasticity. PLoS One, Volume 8 (Number 4). Article number e62509. doi:10.1371/journal.pone.0062509 ISSN 1932-6203.
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WRAP_Palygin_pone.0062509.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1789Kb) | Preview |
Official URL: http://dx.doi.org/10.1371/journal.pone.0062509
Abstract
miR-132 and miR-212 are two closely related miRNAs encoded in the same intron of a small non-coding gene, which have been suggested to play roles in both immune and neuronal function. We describe here the generation and initial characterisation of a miR-132/212 double knockout mouse. These mice were viable and fertile with no overt adverse phenotype. Analysis of innate immune responses, including TLR-induced cytokine production and IFNβ induction in response to viral infection of primary fibroblasts did not reveal any phenotype in the knockouts. In contrast, the loss of miR-132 and miR-212, while not overtly affecting neuronal morphology, did affect synaptic function. In both hippocampal and neocortical slices miR-132/212 knockout reduced basal synaptic transmission, without affecting paired-pulse facilitation. Hippocampal long-term potentiation (LTP) induced by tetanic stimulation was not affected by miR-132/212 deletion, whilst theta burst LTP was enhanced. In contrast, neocortical theta burst-induced LTP was inhibited by loss of miR-132/212. Together these results indicate that miR-132 and/or miR-212 play a significant role in synaptic function, possibly by regulating the number of postsynaptic AMPA receptors under basal conditions and during activity-dependent synaptic plasticity.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Genetics -- Research, Immunogenetics, RNA, Molecular biology -- Research, Neurons | ||||
Journal or Publication Title: | PLoS One | ||||
Publisher: | Public Library of Science | ||||
ISSN: | 1932-6203 | ||||
Official Date: | 26 April 2013 | ||||
Dates: |
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Volume: | Volume 8 | ||||
Number: | Number 4 | ||||
Page Range: | Article number e62509 | ||||
DOI: | 10.1371/journal.pone.0062509 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 24 December 2015 | ||||
Date of first compliant Open Access: | 24 December 2015 | ||||
Funder: | Medical Research Council (Great Britain) (MRC), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Wellcome Trust (London, England), Sixth Framework Programme (European Commission) (FP6) |
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