The Library
Multi-locus sequence analysis, taxonomic resolution and biogeography of marine Synechococcus
Tools
Tools
Tools
Mazard, Sophie, Ostrowski, Martin, Partensky, Frédéric and Scanlan, David J. (2012) Multi-locus sequence analysis, taxonomic resolution and biogeography of marine Synechococcus. Environmental Microbiology, Volume 14 (Number 2). pp. 372-386. doi:10.1111/j.1462-2920.2011.02514.x ISSN 1462-2912.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1111/j.1462-2920.2011.02514.x
Abstract
Conserved markers such as the 16S rRNA gene do not provide sufficient molecular resolution to identify spatially structured populations of marine Synechococcus, or ‘ecotypes’ adapted to distinct ecological niches. Multi-locus sequence analysis targeting seven ‘core’ genes was employed to taxonomically resolve Synechococcus isolates and correlate previous phylogenetic analyses encompassing a range of markers. Despite the recognized importance of lateral gene transfer in shaping the genomes of marine cyanobacteria, multi-locus sequence analysis of more than 120 isolates reflects a clonal population structure of major lineages and subgroups. A single core genome locus, petB, encoding the cytochrome b6 subunit of the cytochrome b6f complex, was selected to expand our understanding of the diversity and ecology of marine Synechococcus populations. Environmental petB sequences cloned from contrasting sites highlight numerous genetically and ecologically distinct clusters, some of which represent novel, environmentally abundant clades without cultured representatives. With a view to scaling ecological analyses, the short sequence, taxonomic resolution and accurate automated alignment of petB is ideally suited to high-throughput and high-resolution sequencing projects to explore links between the ecology, evolution and biology of marine Synechococcus.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
| Journal or Publication Title: | Environmental Microbiology | ||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
| ISSN: | 1462-2912 | ||||
| Official Date: | February 2012 | ||||
| Dates: |
|
||||
| Volume: | Volume 14 | ||||
| Number: | Number 2 | ||||
| Page Range: | pp. 372-386 | ||||
| DOI: | 10.1111/j.1462-2920.2011.02514.x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
