Nusca, T. D., Kim, Y., Maltseva, N., Lee, J. Y., Eschenfeldt, W., Stols, L., Schofield, M. M., Scaglione, J. B., Dixon, S. D., Oves-Costales, Daniel, Challis, G. L., Hanna, P. C., Pfleger, B. F., Joachimiak, A. and Sherman, D. H. (2012) Functional and structural analysis of the siderophore synthetase AsbB through reconstitution of the petrobactin biosynthetic pathway from bacillus anthracis. Journal of Biological Chemistry, Volume 287 (Number 19). pp. 16058-16072. doi:10.1074/jbc.M112.359349 ISSN 0021-9258.

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Abstract

Background: asbABCDEF mediates petrobactin production and facilitates anthrax virulence.

Results: Purified AsbA-E proteins reconstituted petrobactin assembly in vitro. The crystal structure and enzymatic studies of AsbB highlight its function and role in the siderophore pathway.

Conclusion: AsbB characterization demonstrated reaction flexibility and substrate positions in the binding pocket.

Significance: Siderophore synthetases represent promising antimicrobial targets, and characterization of these versatile enzymes enables creation of novel compounds.

Item Type:	Journal Article
Divisions:	Faculty of Science, Engineering and Medicine > Science > Chemistry
Journal or Publication Title:	Journal of Biological Chemistry
Publisher:	American Society for Biochemistry and Molecular Biology
ISSN:	0021-9258
Official Date:	4 May 2012
Dates:	Date Event 4 May 2012 Published
Volume:	Volume 287
Number:	Number 19
Page Range:	pp. 16058-16072
DOI:	10.1074/jbc.M112.359349
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access

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