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Secretory pathway research : the more experimental systems the better
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Denecke, J., Aniento, F., Frigerio, Lorenzo, Hawes, C., Hwang, I., Mathur, J., Neuhaus, J.-M. and Robinson, D. G. (2012) Secretory pathway research : the more experimental systems the better. The Plant Cell, Volume 24 (Number 4). pp. 1316-1326. doi:10.1105/tpc.112.096362 ISSN 1040-4651.
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Official URL: http://dx.doi.org/10.1105/tpc.112.096362
Abstract
Transient gene expression, in plant protoplasts or specific plant tissues, is a key technique in plant molecular cell biology, aimed at exploring gene products and their modifications to examine functional subdomains, their interactions with other biomolecules, and their subcellular localization. Here, we highlight some of the major advantages and potential pitfalls of the most commonly used transient gene expression models and illustrate how ectopic expression and the use of dominant mutants can provide insights into protein function.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | The Plant Cell | ||||
Publisher: | American Society of Plant Biologists | ||||
ISSN: | 1040-4651 | ||||
Official Date: | 2012 | ||||
Dates: |
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Volume: | Volume 24 | ||||
Number: | Number 4 | ||||
Page Range: | pp. 1316-1326 | ||||
DOI: | 10.1105/tpc.112.096362 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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