The Library
Genetic regulation of glucoraphanin accumulation in Beneforté® broccoli
Tools
Traka, Maria H., Saha, Shikha, Huseby, Stine, Kopriva, Stanislav, Walley, Peter Glen, Barker, Guy C., Moore, Jonathan D., Mero, Gene, van den Bosch, Frans, Constant, Howard, Kelly, Leo, Schepers, Hans, Boddupalli, Sekhar and Mithen, Richard F. (2013) Genetic regulation of glucoraphanin accumulation in Beneforté® broccoli. New Phytologist, Volume 198 (Number 4). pp. 1085-1095. doi:10.1111/nph.12232 ISSN 0028-646X.
|
Text
WRAP_Moore_nph12232.pdf - Published Version Download (1698Kb) | Preview |
Official URL: http://dx.doi.org/10.1111/nph.12232
Abstract
Diets rich in broccoli (Brassica oleracea var italica) have been associated with maintenance of cardiovascular health and reduction in risk of cancer. These health benefits have been
attributed to glucoraphanin that specifically accumulates in broccoli. The development of broccoli with enhanced concentrations of glucoraphanin may deliver greater health benefits.
Three high-glucoraphanin F1 broccoli hybrids were developed in independent programmes through genome introgression from the wild species Brassica villosa. Glucoraphanin and other metabolites were quantified in experimental field trials. Global SNP analyses quantified the differential extent of B. villosa introgression
The high-glucoraphanin broccoli hybrids contained 2.5–3 times the glucoraphanin content of standard hybrids due to enhanced sulphate assimilation and modifications in sulphur partitioning between sulphur-containing metabolites. All of the high-glucoraphanin hybrids possessed an introgressed B. villosa segment which contained a B. villosa Myb28 allele. Myb28
expression was increased in all of the high-glucoraphanin hybrids. Two high-glucoraphanin hybrids have been commercialised as Beneforte broccoli.
The study illustrates the translation of research on glucosinolate genetics from Arabidopsis to broccoli, the use of wild Brassica species to develop cultivars with potential consumer benefits, and the development of cultivars with contrasting concentrations of glucoraphanin for use in blinded human intervention studies
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QK Botany |
||||
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre |
||||
Library of Congress Subject Headings (LCSH): | Broccoli -- Research, Nutrition -- Research, Biochemistry -- Research, Genetics -- Research | ||||
Journal or Publication Title: | New Phytologist | ||||
Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
ISSN: | 0028-646X | ||||
Official Date: | June 2013 | ||||
Dates: |
|
||||
Volume: | Volume 198 | ||||
Number: | Number 4 | ||||
Page Range: | pp. 1085-1095 | ||||
DOI: | 10.1111/nph.12232 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Date of first compliant deposit: | 24 December 2015 | ||||
Date of first compliant Open Access: | 24 December 2015 | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC) | ||||
Grant number: | BB/J004545/1 |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |
Downloads
Downloads per month over past year