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Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptideD-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa
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Majce, Vita, Ruane, Karen M., Gobec, Stanislav and Roper, David I. (2013) Crystallization and preliminary X-ray analysis of a UDP-MurNAc-tripeptideD-alanyl-D-alanine-adding enzyme (PaMurF) from Pseudomonas aeruginosa. Acta Crystallographica. Section F: Structural Biology and Crystallization Communications, Volume 69 (number 5). pp. 503-505. doi:10.1107/S1744309113005344 ISSN 1744-3091.
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Official URL: http://dx.doi.org/10.1107/S1744309113005344
Abstract
The ATP-dependent UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase MurF catalyses the last step in the cytoplasmic phase of peptidoglycan biosynthesis, which is critical in the formation of the bacterial cell wall and in the recycling of peptidoglycan intermediates. In this study, the crystallization of MurF from the Gram-negative pathogen Pseudomonas aeruginosa in the presence of its UDP-MurNAc-tripeptide substrate is reported. The crystals belonged to space group P212121, with unit-cell parameters a = 57.81, b = 87.29, c = 92.61 Å, and data were collected to 1.92 Å resolution, allowing study of the enzyme in the substrate-liganded form for the first time.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Acta Crystallographica. Section F: Structural Biology and Crystallization Communications | ||||
Publisher: | Wiley-Blackwell Publishing, Inc. | ||||
ISSN: | 1744-3091 | ||||
Official Date: | May 2013 | ||||
Dates: |
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Volume: | Volume 69 | ||||
Number: | number 5 | ||||
Page Range: | pp. 503-505 | ||||
DOI: | 10.1107/S1744309113005344 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | UNESCO–L’Oreal International Fellowship ‘For Women in Science’, Medical Research Council (MRC), UK Midlands BAG | ||||
Grant number: | G1001023, G1100127 (MRC); DLS proposal MX-8359 (UK Midlands BAG) |
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