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Considerations of noise and measurement reproducibility of circular dichroism measurements using Na[CoIII(EDDS)]
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Chmel, Nikola Paul, Scott, Peter and Rodger, Alison (2012) Considerations of noise and measurement reproducibility of circular dichroism measurements using Na[CoIII(EDDS)]. Chirality, Volume 24 (Number 9). pp. 699-705. doi:10.1002/chir.22005 ISSN 0899-0042.
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Official URL: http://dx.doi.org/10.1002/chir.22005
Abstract
Although circular dichroism (CD) spectroscopy has become ubiquitous in biological, chemical, and pharmacological laboratories, several issues with instrument calibration and standardization, measurements quality, and reproducibility still remain. In the context of validating and developing a wide-wavelength-range stable standard for CD that is available in both enantiomeric forms, (R,R)- and (S,S)-Na[CoIII(EDDS)] (where EDDS is ethylenediaminedisuccinato), we have become aware of sources of error in CD spectroscopy in addition to instrument calibration and user issues. This article summarizes those errors and suggests procedures for minimizing them by averaging over sufficient independent data accumulations with appropriate parameters. Chirality 24:699–705, 2012. © 2012 Wiley Periodicals, Inc.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
Journal or Publication Title: | Chirality | ||||
Publisher: | John Wiley & Sons, Inc. | ||||
ISSN: | 0899-0042 | ||||
Official Date: | 2012 | ||||
Dates: |
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Volume: | Volume 24 | ||||
Number: | Number 9 | ||||
Page Range: | pp. 699-705 | ||||
DOI: | 10.1002/chir.22005 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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