The Library
High-resolution mapping of a fruit firmness-related quantitative trait locus in tomato reveals epistatic interactions associated with a complex combinatorial locus
Tools
Chapman, N. H., Bonnet, J., Grivet, L., Lynn, J., Graham, N., Smith, R., Sun, Guangai, Walley, Peter Glen, Poole, M., Causse, M., King, G. J., Baxter, C. and Seymour, G. B. (Graham B.) (2012) High-resolution mapping of a fruit firmness-related quantitative trait locus in tomato reveals epistatic interactions associated with a complex combinatorial locus. Plant Physiology, Volume 159 (Number 4). pp. 1644-1657. doi:10.1104/pp.112.200634 ISSN 0032-0889.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1104/pp.112.200634
Abstract
Fruit firmness in tomato (Solanum lycopersicum) is determined by a number of factors including cell wall structure, turgor and cuticle properties. Firmness is a complex polygenic trait involving the co-regulation of many genes and has proved especially challenging to unravel. In this study a Quantitative trait locus (QTL) for fruit firmness was mapped to tomato chromosome 2 using the Zamir S. pennellii interspecific introgression (IL) lines and fine mapped in a population consisting of 7500 F2 and F3 lines from IL 2-3 and IL 2-4. This firmness QTL contained five distinct sub-peaks, Firs.p.QTL2.1-2.5 and an effect on a distal region of IL 2-4 that was non-overlapping with IL 2-3. All these effects were located within an 8.6 Mb region. Using genetic markers, each sub-peak within this combinatorial locus was mapped to a physical location within the genome and an ethylene response factor (ERF) underlying Firs.p.QTL2.2 and a region contain three pectin methylesterase (PME) genes underlying Firs.p.QTL2.5 were nominated as QTL candidate genes. Statistical models used to explain the observed variability between lines indicated that these candidates and the non-overlapping portion of IL 2-4 were sufficient to account for the majority of the fruit firmness effects. QRT-PCR was used to quantify expression of each candidate gene. The ERF showed increased expression associated with soft fruit texture in the mapping population. In contrast, PME expression was tightly linked with firm fruit texture. Analysis of a range of recombinant lines revealed evidence for an epistatic interaction that was associated with this combinatorial locus.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Plant Physiology | ||||
Publisher: | American Society of Plant Biologists | ||||
ISSN: | 0032-0889 | ||||
Official Date: | August 2012 | ||||
Dates: |
|
||||
Volume: | Volume 159 | ||||
Number: | Number 4 | ||||
Page Range: | pp. 1644-1657 | ||||
DOI: | 10.1104/pp.112.200634 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |