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Isolation of bacterial strains able to metabolize lignin from screening of environmental samples
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Taylor, C. R. (Charles R.), Hardiman, E. M., Ahmad, Mussawar, Sainsbury, P. D., Norris, Paul R. and Bugg, Tim (2012) Isolation of bacterial strains able to metabolize lignin from screening of environmental samples. Journal of Applied Microbiology, Volume 113 (Number 3). pp. 521-530. doi:10.1111/j.1365-2672.2012.05352.x ISSN 1364-5072.
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Official URL: http://dx.doi.org/10.1111/j.1365-2672.2012.05352.x
Abstract
Aims
To develop a method to detect bacteria from environmental samples that are able to metabolize lignin.
Methods and Results
A previously developed UV–vis assay method for lignin degradation activity has been developed for use as a spray assay on agar plates. Nine mesophilic strains were isolated using this method from woodland soil incubated in enrichment cultures containing wheat straw lignocellulose: four Microbacterium isolates, two Micrococcus isolates, Rhodococcus erythropolis (all Actinobacteria) and two Ochrobactrum isolates (Alphaproteobacteria). Three thermotolerant isolates were isolated from the same screening method applied at 45°C to samples of composted wheat straw from solid-state fermentation: Thermobifida fusca and two isolates related to uncharacterized species of Rhizobiales and Sphingobacterium (Bacteroidetes), the latter strain showing tenfold higher lignin degradation activity than other isolates. The isolated strains were able to depolymerize samples of size-fractionated high molecular weight and low molecular weight Kraft lignin, and produced low molecular weight metabolites oxalic acid and protocatechuic acid from incubations containing wheat straw lignocellulose.
Conclusions
A new method for the isolation of bacteria able to metabolize lignin has been developed, which has been used to identify 12 bacterial isolates from environmental sources. The majority of isolates cluster into the Actinobacteria and the Alphaproteobacteria.
Significance and Impact of the Study
Lignin-degrading bacterial strains could be used to convert lignin-containing feedstocks into renewable chemicals and to identify new bacterial lignin-degrading enzymes.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Journal or Publication Title: | Journal of Applied Microbiology | ||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
| ISSN: | 1364-5072 | ||||
| Official Date: | 2012 | ||||
| Dates: |
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| Volume: | Volume 113 | ||||
| Number: | Number 3 | ||||
| Page Range: | pp. 521-530 | ||||
| DOI: | 10.1111/j.1365-2672.2012.05352.x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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