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Bacterial genome mining for novel natural product discovery
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Song, Lijiang, Laureti, L., Gomez-Escribano, J. P., Fox, David J., Yeo, V., Corre, Christophe, Huang, S., Leblond, P., Aigle, B., Bibb, M. and Challis, Gregory L. (2012) Bacterial genome mining for novel natural product discovery. Planta Medica, Volume 78 (Number 11). Article number IL7. doi:10.1055/s-0032-1320194 ISSN 0032-0943.
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Official URL: http://dx.doi.org/10.1055/s-0032-1320194
Abstract
Bioinformatics analyses have identified gene clusters encoding cryptic polyketide biosynthetic pathways, not associated with the production of known metabolites, in several actinomycete genome sequences. Discovery of the metabolic products of such cryptic gene clusters promises to unearth a hitherto untapped wealth of novel bioactive compounds. However, a major obstacle to the discovery of novel natural products by genome mining is that many cryptic pathways are expressed poorly or not at all under normal laboratory conditions. The discovery of the stambomycins, a new family of 51-membered macrolides with promising anti-cancer activity, as the products of a novel type I modular polyketide synthase (PKS) system identified in the partial genome sequence of Streptomyces ambofaciens will be described. Activation of expression of the pathway by genetic manipulation of a putative pathway-specific regulatory gene was key to this discovery. The structures of these novel macrolides suggests that their biosynthesis involves novel features, including in trans hydroxylation during polyketide chain assembly to provide the hydroxyl group required for offloading of the fully-assembled polyketide chain from the PKS via macrocyclisation. Experiments aimed at probing this unusual transformation will be described.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Engineering > WMG (Formerly the Warwick Manufacturing Group) | ||||
| Journal or Publication Title: | Planta Medica | ||||
| Publisher: | Georg Thieme Verlag | ||||
| ISSN: | 0032-0943 | ||||
| Official Date: | 2012 | ||||
| Dates: |
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| Volume: | Volume 78 | ||||
| Number: | Number 11 | ||||
| Page Range: | Article number IL7 | ||||
| DOI: | 10.1055/s-0032-1320194 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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