The Library
Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy
Tools
Tools
Tools
Pache, Christophe, Bocchio, Noelia L., Bouwens, Arno, Villiger, Martin, Berclaz, Corinne, Goulley, Joan, Gibson, Matthew I., Santschi, Christian and Lasser, Theo (2012) Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy. Optics Express, Volume 20 (Number 19). 21385-21399 . doi:10.1364/OE.20.021385
Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1364/OE.20.021385
Abstract
We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed inherent to OCM. We report on the detection of single 40 nm gold particles with a 0.5 μm lateral and 2 μm axial resolution over a 50 μm depth of field and the three-dimensional localization of gold colloids within living cells. In combination with intrinsic sample contrast measured with dark-field OCM, poli-OCM offers a versatile platform for functional cell imaging.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science > Chemistry | ||||
| Journal or Publication Title: | Optics Express | ||||
| Publisher: | Optical Society of America | ||||
| ISSN: | 1094-4087 | ||||
| Official Date: | 10 September 2012 | ||||
| Dates: |
|
||||
| Volume: | Volume 20 | ||||
| Number: | Number 19 | ||||
| Page Range: | 21385-21399 | ||||
| DOI: | 10.1364/OE.20.021385 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | European Commission within the 7th Framework Programme (FP7), Swiss National Science Foundation (SNSF) | ||||
| Grant number: | FP7-222980 (FP7); S205321L-135353/1 (SNSF) |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
