Sivakumar, Kavitha, Tan, Bee K., Bari, Muhammad F., Vatish, Manu and Randeva, Harpal S. (2013) Lower cerebrospinal fluid/plasma fibroblast growth factor 21 (FGF21) ratios and placental FGF21 production in gestational diabetes. PLoS One, Volume 8 (Number 6). e65254. doi:10.1371/journal.pone.0065254

Preview

Text WRAP_Tan_journal.pone.0065254.pdf - Published Version Available under License Creative Commons Attribution. Download (380Kb) | Preview

Request Changes to record.

Abstract

Objectives: Circulating Fibroblast Growth Factor 21 (FGF21) levels are increased in insulin resistant states such as obesity, type 2 diabetes mellitus and gestational diabetes mellitus (GDM). In addition, GDM is associated with serious maternal and fetal complications. We sought to study human cerebrospinal fluid (CSF) and corresponding circulating FGF21 levels in women with gestational diabetes mellitus (GDM) and in age and BMI matched control subjects. We also assessed FGF21 secretion from GDM and control human placental explants.
Design: CSF and corresponding plasma FGF21 levels of 24 women were measured by ELISA [12 GDM (age: 26–47 years, BMI: 24.3–36.3 kg/m2) and 12 controls (age: 22–40 years, BMI: 30.1–37.0 kg/m2)]. FGF21 levels in conditioned media were secretion from GDM and control human placental explants were also measured by ELISA.
Results: Glucose, HOMA-IR and circulating NEFA levels were significantly higher in women with GDM compared to control subjects. Plasma FGF21 levels were significantly higher in women with GDM compared to control subjects [234.3 (150.2–352.7) vs. 115.5 (60.5–188.7) pg/ml; P<0.05]. However, there was no significant difference in CSF FGF21 levels in women with GDM compared to control subjects. Interestingly, CSF/Plasma FGF21 ratio was significantly lower in women with GDM compared to control subjects [0.4 (0.3–0.6) vs. 0.8 (0.5–1.6); P<0.05]. FGF21 secretion into conditioned media was significantly lower in human placental explants from women with GDM compared to control subjects (P<0.05).
Conclusions: The central actions of FGF21 in GDM subjects maybe pivotal in the pathogenesis of insulin resistance in GDM subjects. The significance of FGF21 produced by the placenta remains uncharted and maybe crucial in our understanding of the patho-physiology of GDM and its associated maternal and fetal complications. Future research should seek to elucidate these points.

Item Type:	Journal Article
Subjects:	R Medicine > R Medicine (General) R Medicine > RG Gynecology and obstetrics R Medicine > RJ Pediatrics
Divisions:	Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Medicine > Warwick Medical School
Library of Congress Subject Headings (LCSH):	Diabetes in pregnancy, Pregnancy -- Complications , Obesity in women , Cerebrospinal fluid, Fibroblast growth factors, Fetus -- Development, Pregnancy, Diabetes
Journal or Publication Title:	PLoS One
Publisher:	Public Library of Science
ISSN:	1932-6203
Official Date:	3 June 2013
Dates:	Date Event 3 June 2013 Published
Date of first compliant deposit:	24 December 2015
Volume:	Volume 8
Number:	Number 6
Page Range:	e65254
DOI:	10.1371/journal.pone.0065254
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Open Access
Funder:	General Charities of the City of Coventry (GCCC)

Request changes or add full text files to a record

Repository staff actions (login required)

View Item

Downloads