The Library
Following vegetative to embryonic cellular changes in leaves of arabidopsis thaliana over-expressing LEAFY COTYLEDON2
Tools
Feeney, Mistianne, Frigerio, Lorenzo, Cui, Y. and Menassa, Rima (2013) Following vegetative to embryonic cellular changes in leaves of arabidopsis thaliana over-expressing LEAFY COTYLEDON2. Plant Physiology, Volume 162 (Number 4). pp. 1881-1896. doi:10.1104/pp.113.220996 ISSN 0032-0889.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1104/pp.113.220996
Abstract
Embryogenesis in flowering plants is controlled by a complex interplay of genetic, biochemical and physiological regulators. LEAFY COTYLEDON2 (LEC2) is among a small number of key transcriptional regulators that are known to play important roles in controlling major events during the maturation stage of embryogenesis, notably, the synthesis and accumulation of storage reserves. LEC2 over-expression causes vegetative tissues to change their developmental fate to an embryonic state however little information exists about the cellular changes that take place. We show that LEC2 alters leaf morphology and anatomy and causes embryogenic structures to form subcellularly in leaves of Arabidopsis thaliana. Chloroplasts accumulate more starch, the cytoplasm fills with oil bodies and lytic vacuoles (LVs) appear smaller in size and accumulate protein deposits. Since LEC2 is responsible for activating the synthesis of seed storage proteins (SSPs) during seed development, SSP accumulation was investigated in leaves. The major Arabidopsis SSP families were shown to accumulate within small leaf vacuoles. By exploiting the developmental and tissue specific localization of two tonoplast intrinsic protein (TIP) isoforms, the small leaf vacuoles were identified as protein storage vacuoles (PSVs). Confocal analyses of leaf vacuoles expressing fluorescently labeled TIP isoforms reveal an altered tonoplast morphology resembling an amalgamation of a LV and PSV. Results suggest that as the LV transitions to a PSV, the tonoplast remodels before the large vacuole lumen is replaced by smaller PSVs. Finally, using vegetative and seed markers to monitor the transition, we show that LEC2 induces a reprogramming of leaf development.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Journal or Publication Title: | Plant Physiology | ||||
Publisher: | American Society of Plant Biologists | ||||
ISSN: | 0032-0889 | ||||
Official Date: | August 2013 | ||||
Dates: |
|
||||
Volume: | Volume 162 | ||||
Number: | Number 4 | ||||
Page Range: | pp. 1881-1896 | ||||
DOI: | 10.1104/pp.113.220996 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | A-base Funding Program of Agriculture 750 and Agri-Food Canada, Leverhulme Trust | ||||
Grant number: | RPG-327 (Leverhulme Trust) |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |