Silver, Barry R. (2013) New approaches to protein crystallization. PhD thesis, University of Warwick.

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Abstract

This thesis was focussed toward providing new approaches to protein
crystallization. Herein, we describe three new such approaches.
Firstly, we describe proof-of-concept studies which demonstrate that simple
DC electrochemical systems may be used to enhance and/or control the growth of
lysozyme protein crystals on the surface of platinum disc electrodes. Secondly, we
demonstrate how various oil/water interfaces provide both novel and unique
environments for the study and enhancement of protein crystallization studies. In
particular, we show how some oil/water interfaces greatly enhance the extent of
lysozyme crystallization in comparison to the air/water interface whilst others do not.
Thirdly, we show for the first time, that by application of small magnitude potentials
to the ITIES, large increases in lysozyme crystal growth can be achieved (on the
ITIES) in short time.
Additionally, and unrelated to protein crystallization, we find that large
potential-dependent changes in surface tension may be achieved by probable
reversible adsorption/desorption of proteins to and from the oil-water interface. The
reversible changes in droplet geometry are, in some cases, large and seem
controllable. As such, this methodology warrants consideration as means to enhance
the performance of alternative liquid/liquid ultra low-voltage and conventional
electrowetting systems. The rapid crystallization of TBATPB at the ITIES is also reported for the first
time. This work is of importance to theory and experiment regarding ion-transfer
mechanisms at the ITIES. Additionally, this work may point towards a new type of
crystallization technology for a variety of molecules grounded in methodology
developed for liquid/liquid electrochemical systems.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QD Chemistry
Library of Congress Subject Headings (LCSH):	Crystallization, Proteins -- Purification, Electrochemistry
Official Date:	2013
Dates:	Date Event 2013 Submitted
Institution:	University of Warwick
Theses Department:	Department of Chemistry
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Unwin, Patrick R.
Extent:	xxv, 301 leaves : illustrations.
Language:	eng

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