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Identification of active methylotrophic bacteria inhabiting surface sediment of a marine estuary
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Moussard, Hélène, Stralis-Pavese, Nancy, Bodrossy, Levente, Neufeld, Josh D. and Murrell, J. C. (J. Colin) (2009) Identification of active methylotrophic bacteria inhabiting surface sediment of a marine estuary. Environmental Microbiology Reports, Vol.1 (No.5). pp. 424-433. doi:10.1111/j.1758-2229.2009.00063.x ISSN 1758-2229.
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Official URL: http://dx.doi.org/10.1111/j.1758-2229.2009.00063.x
Abstract
Methylotrophs play an essential role in the global carbon cycle due to their participation in methane oxidation and C-1 metabolism. Despite this important biogeochemical role, marine and estuarine microorganisms that consume C-1 compounds are poorly characterized. In this study, we investigated the diversity of active methylotrophs and methanotrophs in sediment from the Colne Estuary (Brightlingsea, UK). Aerobic surface sediment samples were examined for the presence of C-1-utilizing communities using DNA stable-isotope probing (DNA-SIP) with 13C-labelled methane, methanol and monomethylamine. Active methylotrophic bacteria were confirmed after DNA-SIP and denaturing gradient gel electrophoresis analyses. Clone libraries of 16S rRNA gene amplicons revealed the presence of methylotrophic bacteria affiliated with Methylophaga spp. in methanol and monomethylamine incubations. The addition of marine ammonium mineral salts medium to the microcosms increased the rate of substrate metabolism in DNA-SIP incubations, although nutrient addition did not affect the active populations contributing 13C-labelled DNA. The 13CH(4) SIP incubations indicated the predominant activity of type I methanotrophs and microarray hybridization of amplified particulate methane monooxygenase (pmoA) genes confirmed the role of type Ia methanotrophs in SIP incubations. Type II methanotrophs (i.e. Methylocystis and Methylosinus) were only detected in the original sediment and in the unlabelled DNA fractions, which indicated that type II methanotrophs were not actively involved in C-1 compound assimilation in DNA-SIP incubations with estuarine surface sediment samples.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) > Biological Sciences ( -2010) | ||||
| Journal or Publication Title: | Environmental Microbiology Reports | ||||
| Publisher: | Wiley-Blackwell Publishing Ltd. | ||||
| ISSN: | 1758-2229 | ||||
| Official Date: | October 2009 | ||||
| Dates: |
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| Volume: | Vol.1 | ||||
| Number: | No.5 | ||||
| Number of Pages: | 10 | ||||
| Page Range: | pp. 424-433 | ||||
| DOI: | 10.1111/j.1758-2229.2009.00063.x | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | Natural Environment Research Council Aquatic Microbial Metagenomics and Biogeochemocal Cycles, Natural Sciences and Engineering Research Council of Canada | ||||
| Grant number: | NE/C001 923/1 |
Data sourced from Thomson Reuters' Web of Knowledge
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