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Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b overexpression enhances water productivity, resistance to drought, and infection
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Bechtold, Ulrike, Albihlal, Waleed S., Lawson, Tracy, Fryer, Michael J., Sparrow, Penelope A. C., Richard, François, Persad, Ramona, Bowden, Laura, Hickman, Richard D. G., Martin, Cathie, Beynon, Jim, Buchanan-Wollaston, Vicky, Baker, N. R., Morison, James I. L., Schoffl, Friedrich, Ott, Sascha and Mullineaux, Philip M. (2013) Arabidopsis HEAT SHOCK TRANSCRIPTION FACTORA1b overexpression enhances water productivity, resistance to drought, and infection. Journal of Experimental Botany, Volume 64 (Number 11). pp. 3467-3481. doi:10.1093/jxb/ert185
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WRAP_Hickman_J. Exp. Bot.-2013-Bechtold-3467-81.pdf - Published Version Available under License Creative Commons Attribution Non-commercial. Download (1848Kb) | Preview |
Official URL: http://dx.doi.org/10.1093/jxb/ert185
Abstract
Heat-stressed crops suffer dehydration, depressed growth, and a consequent decline in water productivity, which is the yield of harvestable product as a function of lifetime water consumption and is a trait associated with plant growth and development. Heat shock transcription factor (HSF) genes have been implicated not only in thermotolerance but also in plant growth and development, and therefore could influence water productivity. Here it is demonstrated that Arabidopsis thaliana plants with increased HSFA1b expression showed increased water productivity and harvest index under water-replete and water-limiting conditions. In non-stressed HSFA1b-overexpressing (HSFA1bOx) plants, 509 genes showed altered expression, and these genes were not over-represented for development-associated genes but were for response to biotic stress. This confirmed an additional role for HSFA1b in maintaining basal disease resistance, which was stress hormone independent but involved H2O2 signalling. Fifty-five of the 509 genes harbour a variant of the heat shock element (HSE) in their promoters, here named HSE1b. Chromatin immunoprecipitation-PCR confirmed binding of HSFA1b to HSE1b in vivo, including in seven transcription factor genes. One of these is MULTIPROTEIN BRIDGING FACTOR1c (MBF1c). Plants overexpressing MBF1c showed enhanced basal resistance but not water productivity, thus partially phenocopying HSFA1bOx plants. A comparison of genes responsive to HSFA1b and MBF1c overexpression revealed a common group, none of which harbours a HSE1b motif. From this example, it is suggested that HSFA1b directly regulates 55 HSE1b-containing genes, which control the remaining 454 genes, collectively accounting for the stress defence and developmental phenotypes of HSFA1bOx.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QK Botany S Agriculture > SB Plant culture |
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| Divisions: | Faculty of Science > Life Sciences (2010- ) Faculty of Science > Centre for Systems Biology |
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| Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana, Crops -- Drought tolerance , Plants -- Effect of heat on, Plant molecular genetics, Plants -- Effect of stress on -- Molecular aspects | ||||
| Journal or Publication Title: | Journal of Experimental Botany | ||||
| Publisher: | OUP | ||||
| ISSN: | 0022-0957 | ||||
| Official Date: | 4 July 2013 | ||||
| Dates: |
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| Date of first compliant deposit: | 25 December 2015 | ||||
| Volume: | Volume 64 | ||||
| Number: | Number 11 | ||||
| Page Range: | pp. 3467-3481 | ||||
| DOI: | 10.1093/jxb/ert185 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access | ||||
| Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), University of Essex | ||||
| Grant number: | BB/F005822/1, BB/F0F/231 (BBSRC) |
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