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LIFEGUARD proteins support plant colonization by biotrophic powdery mildew fungi
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Weis, Corina, Huckelhoven, Ralph and Eichmann, Ruth (2013) LIFEGUARD proteins support plant colonization by biotrophic powdery mildew fungi. Journal of Experimental Botany, Volume 64 (Number 12). pp. 3855-3867. doi:10.1093/jxb/ert217 ISSN 0022-0957.
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WRAP_J. Exp. Bot.-2013-Weis-3855-67.pdf - Published Version Available under License Creative Commons Attribution. Download (2935Kb) | Preview |
Official URL: http://dx.doi.org/10.1093/jxb/ert217
Abstract
Pathogenic microbes manipulate eukaryotic cells during invasion and target plant proteins to achieve host susceptibility. BAX INHIBITOR-1 (BI-1) is an endoplasmic reticulum-resident cell death suppressor in plants and animals and is required for full susceptibility of barley to the barley powdery mildew fungus Blumeria graminis f.sp. hordei. LIFEGUARD (LFG) proteins resemble BI-1 proteins in terms of predicted membrane topology and cell-death-inhibiting function in metazoans, but display clear sequence-specific distinctions. This work shows that barley (Hordeum vulgare L.) and Arabidopsis thaliana genomes harbour five LFG genes, HvLFGa–HvLFGe and AtLFG1–AtLFG5, whose functions are largely uncharacterized. As observed for HvBI-1, single-cell overexpression of HvLFGa supports penetration success of B. graminis f.sp. hordei into barley epidermal cells, while transient-induced gene silencing restricts it. In penetrated barley epidermal cells, a green fluorescent protein-tagged HvLFGa protein accumulates at the site of fungal entry, around fungal haustoria and in endosomal or vacuolar membranes. The data further suggest a role of LFG proteins in plant–powdery mildew interactions in both monocot and dicot plants, because stable overexpression or knockdown of AtLFG1 or AtLFG2 also support or delay development of the powdery mildew fungus Erysiphe cruciferarum on the respective Arabidopsis mutants. Together, this work has identified new modulators of plant–powdery mildew interactions, and the data further support functional similarities between BI-1 and LFG proteins beyond cell death regulation.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QK Botany |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Arabidopsis thaliana, Pathogenic bacteria , Eukaryotic cells -- Genetics, Plant cytogenetics , Plant genomes | ||||
Journal or Publication Title: | Journal of Experimental Botany | ||||
Publisher: | OUP | ||||
ISSN: | 0022-0957 | ||||
Official Date: | 12 September 2013 | ||||
Dates: |
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Volume: | Volume 64 | ||||
Number: | Number 12 | ||||
Page Range: | pp. 3855-3867 | ||||
DOI: | 10.1093/jxb/ert217 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 25 December 2015 | ||||
Date of first compliant Open Access: | 25 December 2015 | ||||
Funder: | Deutsche Forschungsgemeinschaft (DFG) | ||||
Grant number: | EI835/1-1 |
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