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Detection of oxidized and glycated proteins in clinical samples using mass spectrometry — A user's perspective
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Thornalley, Paul J. and Rabbani, Naila (2001) Detection of oxidized and glycated proteins in clinical samples using mass spectrometry — A user's perspective. Biochimica et Biophysica Acta (BBA) - General Subjects, Volume 1840 (Number 2). pp. 1818-829. doi:10.1016/j.bbagen.2013.03.025
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Official URL: http://dx.doi.org/10.1016/j.bbagen.2013.03.025
Abstract
Background
Proteins in human tissues and body fluids continually undergo spontaneous oxidation and glycation reactions forming low levels of oxidation and glycation adduct residues. Proteolysis of oxidised and glycated proteins releases oxidised and glycated amino acids which, if they cannot be repaired, are excreted in urine.
Scope of Review
In this review we give a brief background to the classification, formation and processing of oxidised and glycated proteins in the clinical setting. We then describe the application of stable isotopic dilution analysis liquid chromatography-tandem mass spectrometry (LC-MS/MS) for measurement of oxidative and glycation damage to proteins in clinical studies, sources of error in pre-analytic processing, corroboration with other techniques – including how this may be improved – and a systems approach to protein damage analysis for improved surety of analyte estimations.
Major conclusions
Stable isotopic dilution analysis LC-MS/MS provides a robust reference method for measurement of protein oxidation and glycation adducts. Optimised pre-analytic processing of samples and LC-MS/MS analysis procedures are required to achieve this.
General significance
Quantitative measurement of protein oxidation and glycation adducts provides information on level of exposure to potentially damaging protein modifications, protein inactivation in ageing and disease, metabolic control, protein turnover, renal function and other aspects of body function. Reliable and clinically assessable analysis is required for translation of measurement to clinical diagnostic use. Stable isotopic dilution analysis LC-MS/MS provides a “gold standard” approach and reference methodology to which other higher throughput methods such as immunoassay and indirect methods are preferably corroborated by researchers and those commercialising diagnostic kits and reagents. This article is part of a Special Issue entitled Current methods to study reactive oxygen species - pros and cons.
| Item Type: | Journal Article | ||||||||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Translational & Experimental Medicine > Metabolic and Vascular Health (- until July 2016) Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Biochimica et Biophysica Acta (BBA) - General Subjects | ||||||||||
| Publisher: | Elsevier Science BV | ||||||||||
| ISSN: | 0304-4165 | ||||||||||
| Official Date: | February 2001 | ||||||||||
| Dates: |
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| Volume: | Volume 1840 | ||||||||||
| Number: | Number 2 | ||||||||||
| Page Range: | pp. 1818-829 | ||||||||||
| DOI: | 10.1016/j.bbagen.2013.03.025 | ||||||||||
| Status: | Peer Reviewed | ||||||||||
| Publication Status: | Published | ||||||||||
| Access rights to Published version: | Restricted or Subscription Access |
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