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The AAP gene family for amino acid permeases contributes to development of the cyst nematode Heterodera schachtii in roots of Arabidopsis
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Elashry, Abdelnaser, Okumoto, Sakiko, Siddique, Shahid, Koch, Wolfgang, Kreil, David and Bohlmann, Holger (2013) The AAP gene family for amino acid permeases contributes to development of the cyst nematode Heterodera schachtii in roots of Arabidopsis. Plant Physiology and Biochemistry, Volume 70 . pp. 379-386. doi:10.1016/j.plaphy.2013.05.016 ISSN 0981-9428.
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WRAP_Kreil_1-s2.0-S0981942813001812-main.pdf - Published Version Available under License Creative Commons Attribution. Download (1662Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.plaphy.2013.05.016
Abstract
The beet cyst nematode Heterodera schachtii is able to infect Arabidopsis plants and induce feeding sites in the root. These syncytia are the only source of nutrients for the nematodes throughout their life and are a nutrient sink for the host plant. We have studied here the role of amino acid transporters for nematode development. Arabidopsis contains a large number of different amino acid transporters in several gene families but those of the AAP family were found to be especially expressed in syncytia. Arabidopsis contains 8 AAP genes and they were all strongly expressed in syncytia with the exception of AAP5 and AAP7, which were slightly downregulated. We used promoter::GUS lines and in situ RT-PCR to confirm the expression of several AAP genes and LHT1, a lysine- and histidine-specific amino acid transporter, in syncytia. The strong expression of AAP genes in syncytia indicated that these transporters are important for the transport of amino acids into syncytia and we used T-DNA mutants for several AAP genes to test for their influence on nematode development. We found that mutants of AAP1, AAP2, and AAP8 significantly reduced the number of female nematodes developing on these plants. Our study showed that amino acid transport into syncytia is important for the development of the nematodes.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Sugar beet cyst nematode, Cyst nematodes, Arabidopsis, Amino acids, Plant genetics, Roots (Botany) | ||||
Journal or Publication Title: | Plant Physiology and Biochemistry | ||||
Publisher: | Elsevier France | ||||
ISSN: | 0981-9428 | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | Volume 70 | ||||
Page Range: | pp. 379-386 | ||||
DOI: | 10.1016/j.plaphy.2013.05.016 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Date of first compliant deposit: | 25 December 2015 | ||||
Date of first compliant Open Access: | 25 December 2015 | ||||
Funder: | Fonds zur Förderung der Wissenschaftlichen Forschung (Austria) (FWF), Pakistan. Higher Education Commission, Wiener Wissenschafts, Forschungs und Technologiefonds [Vienna Science and Technology Fund] (WWTF), Austrian Institute of Technology (AIT), Austrian Center of Biopharmaceutical Technology (ACBT) | ||||
Grant number: | P16296-B06, P20471-B11 (FWF) |
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