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Tailoring reactions catalyzed by heme-dependent enzymes
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Barry, Sarah M. and Challis, Gregory L. (2012) Tailoring reactions catalyzed by heme-dependent enzymes. Methods in Enzymology, Volume 516 (Chapter 9). pp. 171-194. doi:10.1016/B978-0-12-394291-3.00001-0
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Official URL: http://dx.doi.org/10.1016/B978-0-12-394291-3.00001...
Abstract
There is a truly vast quantity of research articles and textbooks, aimed at a variety of audiences, on cytochromes P450. However, a large amount of specialized terminology has become associated with these enzymes, which can be daunting to those new to the field. The aim of this chapter is to give a brief overview of the functions and importance of cytochromes P450 with particular emphasis on their roles as tailoring enzymes in natural product biosynthetic pathways. Differences between the biosynthetic enzymes and their catabolic counterparts are highlighted. Assays used to investigate substrate binding to cytochromes P450 are described using TxtE, a recently discovered unique nitrating enzyme involved in thaxtomin A biosynthesis, as an example.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Journal or Publication Title: | Methods in Enzymology | ||||
| Publisher: | Academic Press | ||||
| ISSN: | 0076-6879 | ||||
| Book Title: | Natural Product Biosynthesis by Microorganisms and Plants, Part B | ||||
| Official Date: | 2012 | ||||
| Dates: |
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| Volume: | Volume 516 | ||||
| Number: | Chapter 9 | ||||
| Page Range: | pp. 171-194 | ||||
| DOI: | 10.1016/B978-0-12-394291-3.00001-0 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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