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Alignment of a model amyloid peptide fragment in bulk and at a solid surface

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Hamley, Ian W., Castelletto, Valeria, Moulton, Claire M., Rodriguez-Perez, Jose, Squires, Adam M., Eralp, Tugce, Held, Georg, Hicks, Matthew R. and Rodger, Alison. (2010) Alignment of a model amyloid peptide fragment in bulk and at a solid surface. Journal of Physical Chemistry B, Vol.114 (No.24). pp. 8244-8254. ISSN 1520-6106

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Abstract

The alignment of model amyloid peptide YYKLVFFC is investigated in bulk and at a solid surface using a range of spectroscopic methods employing polarized radiation. The peptide is based on a core sequence of the amyloid beta (A beta) peptide, KLVFF. The attached tyrosine and cysteine units are exploited to yield information on alignment and possible formation of disulfide or dityrosine links. Polarized Raman spectroscopy on aligned stalks provides information on tyrosine orientation, which complements data from linear dichroism (LD) on aqueous solutions subjected to shear in a Couette cell. LD provides a detailed picture of alignment of peptide strands and aromatic residues and was also used to probe the kinetics of self-assembly. This suggests initial association of phenylalanine residues, followed by subsequent registry of strands and orientation of tyrosine residues. X-ray diffraction (XRD) data from aligned stalks is used to extract orientational order parameters from the 0.48 nm reflection in the cross-beta pattern, from which an orientational distribution function is obtained. X-ray diffraction on solutions subject to capillary flow confirmed orientation in situ at the level of the cross-beta pattern. The information on fibril and tyrosine orientation from polarized Raman spectroscopy is compared with results from NEXAFS experiments on samples prepared as films on silicon. This indicates fibrils are aligned parallel to the surface, with phenyl ring normals perpendicular to the surface. Possible disulfide bridging leading to peptide dimer formation was excluded by Raman spectroscopy, whereas dityrosine formation was probed by fluorescence experiments and was found not to occur except under alkaline conditions. Congo red binding was found not to influence the cross-beta XRD pattern.

Item Type: Journal Article
Subjects: Q Science > QD Chemistry
Q Science > QP Physiology
Divisions: Faculty of Science > Chemistry
Library of Congress Subject Headings (LCSH): Amyloid, Amyloid beta-protein, Self-assembly (Chemistry)
Journal or Publication Title: Journal of Physical Chemistry B
Publisher: American Chemical Society
ISSN: 1520-6106
Official Date: 24 June 2010
Volume: Vol.114
Number: No.24
Number of Pages: 11
Page Range: pp. 8244-8254
Identification Number: 10.1021/jp101374e
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Engineering and Physical Sciences Research Council (EPSRC), MAX Laboratory, Seventh Framework Programme (European Commission) (FP7)
Grant number: EP/F048114/1 (EPSRC), EP/G026203/1 (EPSRC), 511/3-261 (ML), FP7/2007-2013 (FP7), 226716 (FP7)
References:

(1) Mata, A.; Hsu, L.; Capito, R.; Aparicio, C.; Henrikson, K.; Stupp,
S. I. Soft Matter 2009, 5, 1228–1236.
(2) Marklein, R. A.; Burdick, J. A. AdV. Mater. 2010, 22, 175–189.
(3) Gras, S. L.; Tickler, A. K.; Squires, A. M.; Devlin, G. L.; Horton,
M. A.; Dobson, C. M.; MacPhee, C. Biomaterials 2008, 29 (11), 1553–
1562.
(4) Hamley, I. W.; Castelletto, V.; Moulton, C. M.; Myatt, D.; Siligardi,
G.; Oliveira, C. L. P.; Pedersen, J. S.; Gavish, I.; Danino, D. Macromol.
Biosci. 2010, 10, 40–48.
(5) Krysmann, M. J.; Castelletto, V.; Kelarakis, A.; Hamley, I. W.;
Hule, R. A.; Pochan, D. J. Biochemistry 2008, 47, 4597–4605.
(6) Ban, H.; Gavrilyuk, J.; Barbas, C. F. J. Am. Chem. Soc. 2010, 132,
1523–1525.
(7) Tsuboi, M.; Ezaki, Y.; Aida, M.; Suzuki, M.; Yimit, A.; Ushizawa,
K.; Ueda, T. Biospectroscopy 1998, 4 (1), 61–71.
(8) Tsuboi, M.; Kubo, Y.; Akahane, K.; Benevides, J. M.; Thomas,
G. J. J. Raman Spectrosc. 2006, 37 (1-3), 240–247.
(9) Rousseau, M. E.; Lefevre, T.; Beaulieu, L.; Asakura, T.; Pezolet,
M. Biomacromolecules 2004, 5 (6), 2247–2257.
(10) Tsuboi, M.; Kubo, Y.; Ikeda, T.; Overman, S. A.; Osman, O.;
Thomas, G. J. Biochemistry 2003, 42 (4), 940–950.
(11) Overman, S. A.; Tsuboi, M.; Thomas, G. J. Biophys. J. 1998, 74
(2), A73–A73.
(12) Polzonetti, G.; Battocchio, C.; Lucci, G.; Dettin, M.; Gambaretto,
R.; Di Bello, C.; Carravetta, V. Mater. Sci. Eng., C 2006, 26 (5-7), 929–
934.
(13) Iucci, G.; Battocchio, C.; Dettin, M.; Gambaretto, R.; Di Bello,
C.; Borgatti, F.; Carravetta, V.; Monti, S.; Polzonetti, G. Surf. Sci. 2007,
601 (18), 3843–3849.
(14) Iucci, G.; Battocchio, C.; Dettin, M.; Gambaretto, R.; Polzonetti,
G. Surf. Interface Anal. 2008, 40 (3-4), 210–214.
(15) Childers, W. S.; Mehta, A. K.; Lu, K.; Lynn, D. G. J. Am. Chem.
Soc. 2009, 131 (29), 10165–10172.
(16) Bulheller, B. M.; Rodger, A.; Hirst, J. D. Phys. Chem. Chem. Phys.
2007, 9 (17), 2020–2035.
(17) Marrington, R.; Dafforn, T. R.; Halsall, D. J.; Rodger, A. Biophys.
J. 2004, 87 (3), 2002–2012.
(18) Rodger, A.; Marrington, R.; Geeves, M. A.; Hicks, M.; de Alwis,
L.; Halsall, D. J.; Dafforn, T. R. Phys. Chem. Chem. Phys. 2006, 8 (27),
3161–3171.
(19) Adachi, R.; Yamaguchi, K.; Yagi, H.; Sakurai, K.; Naiki, H.; Goto,
Y. J. Biol. Chem. 2007, 282 (12), 8978–8983.
(20) Hiramatsu, H.; Goto, Y.; Naiki, H.; Kitagawa, T. J. Am. Chem.
Soc. 2004, 126 (10), 3008–3009.
(21) Hiramatsu, H.; Kitagawa, T. Biochim. Biophys. Acta 2005, 1753,
100–107.
(22) Kim, H. S.; Hartgerink, J. D.; Ghadiri, M. R. J. Am. Chem. Soc.
1998, 120, 4417–4424.
(23) Paramonov, S. E.; Jun, H.-W.; Hartgerink, J. D. J. Am. Chem. Soc.
2006, 128, 7291–7298.
(24) Castelletto, V.; Hamley, I. W.; Harris, P. J. F. Biophys. Chem. 2008,
139, 29–35.
(25) Krysmann, M. J.; Castelletto, V.; Hamley, I. W. Soft Matter 2007,
3, 1401–1406.
(26) Castelletto, V.; Hamley, I. W. Polym. AdV. Technol. 2006, 17 (3),
137–144.
(27) Deutsch, M. Phys. ReV. A 1991, 44 (12), 8264–8270.
(28) Leadbetter, A. J.; Norris, E. K. Mol. Phys. 1979, 38 (3), 669–686.
(29) Leadbetter, A. J., Structural studies of nematic, smectic A and
smectic C phases. In The Molecular Physics of Liquid Crystals; Luckhurst,
G. R., Gray, G. W., Eds.; Academic: London, 1979; pp 286-316.
(30) Tsuboi, M.; Benevides, J. M.; Thomas, G. J. Proc. Jpn. Acad., Ser.
B 2009, 85 (3), 83–97.
(31) Sugeta, H.; Go, A.; Miyazawa, T. Chem. Lett. 1972, 82–86.
(32) Wart, H. E. V.; Lewis, A.; Scheraga, H. A.; Saeva, F. D. Proc.
Natl. Acad. Sci. U.S.A. 1973, 70 (9), 2619–2623.
(33) Qian, W.; Zhao, W.; Krimm, S. J. Mol. Struct. 1991, 250, 89–102.
(34) Li, H.; Hanson, C.; Fuchs, J. A.; Woodward, C.; Thomas, G. J.
Biochemistry 1993, 32, 5800–5808.
(35) Li, H. M.; Thomas, G. J. J. Am. Chem. Soc. 1991, 113 (2), 456–
462.
(36) Raso, S. W.; Clark, P. L.; Haase-Pettingell, C.; King, J.; Thomas,
G. J. J. Mol. Biol. 2001, 307 (3), 899–911.
(37) Rodrı´guez-Pe´rez, J.; Hamley I. W.; Squires, A. M. 2010, in
preparation.
(38) Wei, F.; Zhang, D.; Halas, N. J.; Hartgerink, J. D. J. Phys. Chem.
B 2008, 112, 9158–9164.
(39) Overman, S. A.; Aubrey, K. L.; Vispo, N. S.; Cesareni, G.; Thomas,
G. J. Biochemistry 1994, 33 (5), 1037–1042.
(40) Overman, S. A.; Thomas, G. J. Biochemistry 1995, 34 (23), 7780–
7780.
(41) De Gelder, J.; De Gussem, K.; Vandenabeele, P.; Moens, L. J.
Raman Spectrosc. 2007, 38 (9), 1133–1147.
(42) Lakowicz, J. R., Principles of Fluorescence Spectroscopy, 2nd ed.;
Kluwer: Amsterdam, 1999.
(43) Smail, E. H.; Briza, P.; Panagos, A.; Berenfeld, L. Infect. Immun.
1995, 63 (10), 4078–4083.
(44) Giri, K.; Bhattacharyya, N. P.; Basak, S. Biophys. J. 2007, 92, 293–
302.
(45) Dafforn, T. R.; Rajendra, J.; Halsall, D. J.; Serpell, L. C.; Rodger,
A. Biophys. J. 2004, 86 (1), 404–410.
(46) Du, H.; Fuh, R. C. A.; Li, J. Z.; Corkan, L. A.; Lindsey, J. S.
Photochem. Photobiol. 1998, 68 (2), 141–142.
(47) Marshall, K. E.; Hicks, M. R.; Williams, T. L.; Hoffmann, S. V.;
Rodger, A.; Dafforn, T. R.; Serpell, L. C. Biophys. J. 2010, 98, 330–338.
(48) Perkampus, H.-H., UV-Vis Atlas of Organic Compounds; VCH:
Weinheim, 1992; Vol. 2.
(49) Du, H.; Fuh, R. A.; Li, J.; Corkan, A.; Lindsey, J. S. Photochem.
Photobiol. 1998, 68, 141–142.
(50) Jung, J.-M.; Mezzenga, R. Langmuir 2010, 26, 504–514.
(51) Squires, A. M.; Devlin, G. L.; Gras, S. L.; Tickler, A. K.; MacPhee,
C. E.; Dobson, C. M. J. Am. Chem. Soc. 2006, 128 (36), 11738–11739.
(52) Zhang, S. G.; Holmes, T.; Lockshin, C.; Rich, A. Proc. Natl. Acad.
Sci. U.S.A. 1993, 90 (8), 3334–3338.
(53) Overman, S. A.; Thomas, G. J. Biochemistry 1995, 34 (16), 5440–
5451, erratum 7780.
(54) Overman, S. A.; Thomas, G. J. Biochemistry 1999, 38 (13),
4018–4027.
URI: http://wrap.warwick.ac.uk/id/eprint/5683

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