Promoter diversity in multigene transformation
Peremarti, Ariadna, Twyman, Richard M., Gomez-Galera, Sonia, Naqvi, Shaista, Farre, Gemma, Sabalza, Maite, Miralpeix, Bruna, Dashevskaya, Svetlana, Yuan, Dawei, Ramessar, Koreen, Christou, Paul, Zhu, Changfu, Bassie, Ludovic and Capell, Teresa (2010) Promoter diversity in multigene transformation. Plant Molecular Biology, Vol.73 (No.4-5). pp. 363-378. ISSN 0167-4412Full text not available from this repository.
Official URL: http://dx.doi.org/10.1007/s11103-010-9628-1
Multigene transformation (MGT) is becoming routine in plant biotechnology as researchers seek to generate more complex and ambitious phenotypes in transgenic plants. Every nuclear transgene requires its own promoter, so when coordinated expression is required, the introduction of multiple genes leads inevitably to two opposing strategies: different promoters may be used for each transgene, or the same promoter may be used over and over again. In the former case, there may be a shortage of different promoters with matching activities, but repetitious promoter use may in some cases have a negative impact on transgene stability and expression. Using illustrative case studies, we discuss promoter deployment strategies in transgenic plants that increase the likelihood of successful and stable multiple transgene expression.
|Item Type:||Journal Item|
|Subjects:||Q Science > QD Chemistry
S Agriculture > SB Plant culture
|Divisions:||Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)|
|Journal or Publication Title:||Plant Molecular Biology|
|Number of Pages:||16|
|Page Range:||pp. 363-378|
|Access rights to Published version:||Restricted or Subscription Access|
|Funder:||Ministry of Science and Innovation, Spain, European Research Council Advanced Grant (BIOFORCE), MICINN, Spain|
|Grant number:||BFU2007-61413, BIO2007-30738-E|
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