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The distribution of Dishevelled in convergently extending mesoderm
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Panousopoulou, Eleni, Tyson, Richard A. (Richard Anthony), Bretschneider, Till and Green, Jeremy B.A. (2013) The distribution of Dishevelled in convergently extending mesoderm. Developmental Biology, Volume 382 (Number 2). pp. 496-503. doi:10.1016/j.ydbio.2013.07.012 ISSN 0012-1606.
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WRAP_Tyson_Dishevelled_1-s2.0-S0012160613003801-main.pdf Available under License Creative Commons Attribution. Download (1670Kb) | Preview |
Official URL: http://dx.doi.org/10.1016/j.ydbio.2013.07.012
Abstract
Convergent extension (CE) is a conserved morphogenetic movement that drives axial lengthening of the primary body axis and depends on the planar cell polarity (PCP) pathway. In Drosophila epithelia, a polarised subcellular accumulation of PCP core components, such as Dishevelled (Dvl) protein, is associated with PCP function. Dvl has long been thought to accumulate in the mediolateral protrusions in Xenopus chordamesoderm cells undergoing CE. Here we present a quantitative analysis of Dvl intracellular localisation in Xenopus chordamesoderm cells. We find that, surprisingly, accumulations previously observed at mediolateral protrusions of chordamesodermal cells are not protrusion-specific but reflect yolk-free cytoplasm and are quantitatively matched by the distribution of the cytoplasm-filling lineage marker dextran. However, separating cell cortex-associated from bulk Dvl signal reveals a statistical enrichment of Dvl in notochord–somite boundary-(NSB)-directed protrusions, which is dependent upon NSB proximity. Dvl puncta were also observed, but only upon elevated overexpression. These puncta showed no statistically significant spatial bias, in contrast to the strongly posteriorly-enriched GFP-Dvl puncta previously reported in zebrafish. We propose that Dvl distribution is more subtle and dynamic than previously appreciated and that in vertebrate mesoderm it reflects processes other than protrusion as such.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology | ||||
Divisions: | Faculty of Science, Engineering and Medicine > Research Centres > Warwick Systems Biology Centre | ||||
Library of Congress Subject Headings (LCSH): | Mesoderm, Xenopus, Image analysis, Epithelial cells, Morphogenesis | ||||
Journal or Publication Title: | Developmental Biology | ||||
Publisher: | Academic Press | ||||
ISSN: | 0012-1606 | ||||
Official Date: | 15 October 2013 | ||||
Dates: |
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Volume: | Volume 382 | ||||
Number: | Number 2 | ||||
Page Range: | pp. 496-503 | ||||
DOI: | 10.1016/j.ydbio.2013.07.012 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access | ||||
Funder: | Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), King's College London, Wellcome Trust (London, England) | ||||
Grant number: | BB/D010640/1 (BBSRC) ; WT094131MA (WT) |
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