Sparkes, Imogen, Tolley, Nicholas, Aller, Isabel, Svozil, Julia, Osterrieder, Anne, Botchway, Stanley, Mueller, Christopher, Frigerio, Lorenzo and Hawes, Chris. (2010) Five arabidopsis reticulon isoforms share endoplasmic reticulum location, topology, and membrane-shaping properties. Plant Cell, Vol.22 (No.4). pp. 1333-1343. ISSN 1040-4651

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Abstract

The cortical endoplasmic reticulum (ER) in tobacco (Nicotiana tabacum) epidermal cells is a network of tubules and cisternae undergoing dramatic rearrangements. Reticulons are integral membrane proteins involved in shaping ER tubules. Here, we characterized the localization, topology, effect, and interactions of five Arabidopsis thaliana reticulons (RTNs), isoforms 1-4 and 13, in the cortical ER. Our results indicate that RTNLB13 and RTNLB1-4 colocate to and constrict the tubular ER membrane. All five RTNs preferentially accumulate on ER tubules and are excluded from ER cisternae. All isoforms share the same transmembrane topology, with N and C termini facing the cytosol and four transmembrane domains. We show by Forster resonance energy transfer and fluorescence lifetime imaging microscopy that several RTNs have the capacity to interact with themselves and each other, and we suggest that oligomerization is responsible for their residence in the ER membrane. We also show that a complete reticulon homology domain is required for both RTN residence in high-curvature ER membranes and ER tubule constriction, yet it is not necessary for homotypic interactions.

Item Type:	Journal Article
Subjects:	Q Science > QD Chemistry S Agriculture > SB Plant culture Q Science > QH Natural history > QH301 Biology
Divisions:	Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Journal or Publication Title:	Plant Cell
Publisher:	American Society of Plant Biologists
ISSN:	1040-4651
Date:	April 2010
Volume:	Vol.22
Number:	No.4
Number of Pages:	11
Page Range:	pp. 1333-1343
Identification Number:	10.1105/tpc.110.074385
Status:	Peer Reviewed
Publication Status:	Published
Access rights to Published version:	Restricted or Subscription Access
Funder:	Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Leverhulme Trust (LT), Leverhulme, Heidelberg University, European Union
Grant number:	F/00 382/G, BB/F008147/1, LSH-2002-1.2.5-2, F/00215/AP
URI:	http://wrap.warwick.ac.uk/id/eprint/5760

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