The Library
Differential triggering of spontaneous glutamate release by P/Q-, N- and R-type Ca2+ channels
Tools
Ermolyuk, Yaroslav S., Alder, Felicity G., Surges, Rainer, Pavlov, Ivan Y., Timofeeva, Yulia, Kullmann, Dimitri M. and Volynski, Kirill E. (2013) Differential triggering of spontaneous glutamate release by P/Q-, N- and R-type Ca2+ channels. Nature Neuroscience, Volume 16 (Number 12). pp. 1754-1763. doi:10.1038/nn.3563 ISSN 1097-6256.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1038/nn.3563
Abstract
The role of voltage-gated Ca2+ channels (VGCCs) in spontaneous miniature neurotransmitter release is incompletely understood. We found that stochastic opening of P/Q-, N- and R-type VGCCs accounts for ~50% of all spontaneous glutamate release at rat cultured hippocampal synapses, and that R-type channels have a far greater role in spontaneous than in action potential–evoked exocytosis. VGCC-dependent miniature neurotransmitter release (minis) showed similar sensitivity to presynaptic Ca2+ chelation as evoked release, arguing for direct triggering of spontaneous release by transient spatially localized Ca2+ domains. Experimentally constrained three-dimensional diffusion modeling of Ca2+ influx–exocytosis coupling was consistent with clustered distribution of VGCCs in the active zone of small hippocampal synapses and revealed that spontaneous VGCCs openings can account for the experimentally observed VGCC-dependent minis, although single channel openings triggered release with low probability. Uncorrelated stochastic VGCC opening is therefore a major trigger for spontaneous glutamate release, with differential roles for distinct channel subtypes.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Science > Computer Science | ||||
Journal or Publication Title: | Nature Neuroscience | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 1097-6256 | ||||
Official Date: | 3 November 2013 | ||||
Dates: |
|
||||
Volume: | Volume 16 | ||||
Number: | Number 12 | ||||
Page Range: | pp. 1754-1763 | ||||
DOI: | 10.1038/nn.3563 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |