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Electron transfer dissociation with supplemental activation to differentiate aspartic and isoaspartic residues in doubly charged peptide cations
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Chan, Wai Yi Kelly, Chan, T. W. Dominic and O'Connor, Peter B. (2010) Electron transfer dissociation with supplemental activation to differentiate aspartic and isoaspartic residues in doubly charged peptide cations. Journal of The American Society for Mass Spectrometry, Vol.21 (No.6). pp. 1012-1015. doi:10.1016/j.jasms.2010.02.002 ISSN 1044-0305.
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Official URL: http://dx.doi.org/10.1016/j.jasms.2010.02.002
Abstract
Electron-transfer dissociation (ETD) with supplemental activation of the doubly charged deamidated tryptic digested peptide ions allows differentiation of isoaspartic acid and aspartic acid residues using the c + 57 or z(center dot) - 57 peaks. The diagnostic peak clearly localizes and characterizes the isoaspartic acid residue. Supplemental activation in ETD of the doubly charged peptide ions involves resonant excitation of the charge reduced precursor radical cations and leads to further dissociation, including extra backbone cleavages and secondary fragmentation. Supplemental activation is essential to obtain a high quality ETD spectrum (especially for doubly charged peptide ions) with sequence information. Unfortunately, the low-resolution of the ion trap mass spectrometer makes detection of the diagnostic peak, [M-60], for the aspartic acid residue difficult due to interference with side-chain loss from arginine and glutamic acid residues. (J Am Soc Mass Spectrom 2010, 21, 1012-1015) (C) 2010 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Subjects: | Q Science > QD Chemistry | ||||
| Divisions: | Faculty of Science, Engineering and Medicine > Science > Chemistry | ||||
| Library of Congress Subject Headings (LCSH): | Dissociation, Racemization, Deamination, Isoaspartic acid, Aspartic acid, Mass spectrometry, Peptides | ||||
| Journal or Publication Title: | Journal of The American Society for Mass Spectrometry | ||||
| Publisher: | Springer New York LLC | ||||
| ISSN: | 1044-0305 | ||||
| Official Date: | June 2010 | ||||
| Dates: |
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| Volume: | Vol.21 | ||||
| Number: | No.6 | ||||
| Number of Pages: | 4 | ||||
| Page Range: | pp. 1012-1015 | ||||
| DOI: | 10.1016/j.jasms.2010.02.002 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | National Institutes of Health (U.S.) (NIH), Warwick Centre for Analytical Science, Hong Kong (China). Research Grants Council, Chinese University of Hong Kong | ||||
| Grant number: | GM078293 (NIH), 400207 (HSKAR) |
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