Stott, Andrew James (1990) Studies on human blood platelets. PhD thesis, University of Warwick.

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Abstract

This
project was undertaken to increase the understanding of platelet function, with
paticular emphasis on abnormal platelets in diabetes mellitus, and improving the quality of
platelet concentrates for transfusion.
{1} Potential
platelet antagonists,
including PGE1,
verapamil,
insulin
and
hirudin,
were added to platelet concentrates
in
an attempt to
improve
the recovery and shelf-life of the
concentrate.
Each "preservative"
caused some
improvement in
platelet concentrate quality, as
measured
by functional
tests, and radio-immunoassay
for the platelet activation marker,
B-
thromboglobulin. The best
results were obtained with the addition of
PGE1,
which
facilitated
recovery of all samples to which
it
was added, suggesting a cheap way of ensuring
consistently good platelet concentrates.
{2} Various investigations
were carried out regarding abnormal platelet
function in
diabetes mellitus.
No
significant
differences
were
found in
the responses of platelets
from
diabetics
and age-matched controls to the calcium-channel
blocking drug, Verapamil, in
vitro.
Similarly, the
capacity of
diabetic
platelets to produce malondialdehyde, a
by-product
of thromboxane
A2
synthesis, was not significantly
different from
control platelets.
The
use of insulin
in
aggregometry studies showed, surprisingly, that
insulin
could
have
a pro-aggregatory effect on platelets
from diabetics, but
not
those
from healthy
controls.
In
addition, evidence
for the existence of a platelet aggregation-enhancing
factor in
the
plasma of
diabetics
and older controls was obtained.
{3} Extensive
tests to
investigate
the nature of spontaneous platelet aggregation
(SPA) in
whole
blood have
established the existence of two types of
SPA, (i) ADP-dependent, and
(ii)
ADP-independent. The
results obtained suggest a major role
for
erythrocytes in the
development
of
inappropriate
platelet aggregation.

Item Type:	Thesis or Dissertation (PhD)
Subjects:	Q Science > QD Chemistry R Medicine > R Medicine (General)
Library of Congress Subject Headings (LCSH):	Blood platelets, Diabetes -- Research
Official Date:	September 1990
Dates:	Date Event September 1990 Submitted
Institution:	University of Warwick
Theses Department:	Department of Chemistry
Thesis Type:	PhD
Publication Status:	Unpublished
Supervisor(s)/Advisor:	Swoboda, B. E. P.
Sponsors:	City of Coventry's Charities Commission ; University of Warwick
Extent:	212 leaves : illustrations
Language:	eng

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