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Specificity determinants for lysine incorporation in staphylococcus aureus peptidoglycan as revealed by the structure of a MurE enzyme ternary complex
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Ruane, Karen M., Lloyd, Adrian J., Fülöp, Vilmos, Dowson, Christopher G., Barreteau, Hélène, Boniface, Audrey, Dementin, Sébastien, Blanot, Didier, Mengin-Lecreulx, Dominique, Gobec, Stanislav, Dessen, Andréa and Roper, David I. (2013) Specificity determinants for lysine incorporation in staphylococcus aureus peptidoglycan as revealed by the structure of a MurE enzyme ternary complex. Journal of Biological Chemistry, Volume 288 (Number 46). Article number 33439. doi:10.1074/jbc.M113.508135 ISSN 0021-9258.
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Official URL: http://dx.doi.org/10.1074/jbc.M113.508135
Abstract
Background: MurE controls stereo chemical incorporation of Lysine or diaminopimelate into peptidoglycan stem peptides
Results: The structure of S.aureus MurE reveals an unexpected lack of specificity for Lysine within the active site.
Conclusion: Incorporation of Lysine is supported by the comparatively high concentration of cytoplasmic lysine, not enzyme specificity.
Significance: This study provides new perspectives in targeting Gram-positive peptidoglycan assembly for antimicrobial discovery.
Item Type: | Journal Article | ||||
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Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Science > Life Sciences (2010- ) | ||||
Library of Congress Subject Headings (LCSH): | Staphylococcus aureus, Lysine, Peptidoglycans, Anti-infective agents | ||||
Journal or Publication Title: | Journal of Biological Chemistry | ||||
Publisher: | American Society for Biochemistry and Molecular Biology | ||||
ISSN: | 0021-9258 | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | Volume 288 | ||||
Number: | Number 46 | ||||
Page Range: | Article number 33439 | ||||
DOI: | 10.1074/jbc.M113.508135 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Open Access (Creative Commons) | ||||
Funder: | Medical Research Council (Great Britain) (MRC), Wellcome Trust (London, England), Biotechnology and Biological Sciences Research Council (Great Britain) (BBSRC), Science City Research Alliance | ||||
Grant number: | G500643, G0600801 (MRC) ; 071998, 068598 (WT) ; D524516 |
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