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Monomethylamine as a nitrogen source for a nonmethylotrophic bacterium, Agrobacterium tumefaciens

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Chen, Yin, McAleer, Kathryn L. and Murrell, J. C. (J. Colin). (2010) Monomethylamine as a nitrogen source for a nonmethylotrophic bacterium, Agrobacterium tumefaciens. Applied and Environmental Microbiology, Vol.76 (No.12). pp. 4102-4104. ISSN 0099-2240

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Official URL: http://dx.doi.org/10.1128/AEM.00469-10

Abstract

Monomethylamine can be used by nonmethylotrophs as a sole nitrogen source but not as a carbon source; however, little is known about the genes and enzymes involved. The gamma-glutamylmethylamide/N-methylglutamate pathway for monomethylamine utilization by methylotrophs has recently been resolved. We have identified genes encoding key enzymes of this pathway in nonmethylotrophs (e. g., Agrobacterium tumefaciens) and demonstrated that this pathway is also involved in the utilization of monomethylamine as a nitrogen source by nonmethylotrophs.

Item Type: Journal Article
Subjects: Q Science > QR Microbiology
Divisions: Faculty of Science > Life Sciences (2010- ) > Biological Sciences ( -2010)
Library of Congress Subject Headings (LCSH): Methylamines, Agrobacterium tumefaciens -- Genetics, Agrobacterium tumefaciens -- Physiology
Journal or Publication Title: Applied and Environmental Microbiology
Publisher: American Society for Microbiology
ISSN: 0099-2240
Date: June 2010
Volume: Vol.76
Number: No.12
Number of Pages: 3
Page Range: pp. 4102-4104
Identification Number: 10.1128/AEM.00469-10
Status: Peer Reviewed
Publication Status: Published
Access rights to Published version: Restricted or Subscription Access
Funder: Society for General Microbiology
References: 1. Anthony, C. 1982. The biochemistry of methylotrophs. Academic Press, London. 2. Barrett, E.L., and H. S. Kwan. 1985. Bacterial reduction of trimethylamine oxide. Ann. Rev. Microbiol. 39: 131-149. 3. Bicknell, B., and J. D. Owens. 1980. Utilization of methyl amines as nitrogen sources by non-methylotrophs. J. Gen. Microbiol. 117: 89-96. 4. Brown, C.M., and M.J. Dilworth. 1975. Ammonia assimilation by Rhizobium cultures and bacteroids. J. Gen. Microbiol. 86: 39-48. 5. Budd, J. A., and C. P. Spencer. 1968. The utilisation of alkylated amines by marine bacteria. Mar. Biol. 2: 92-101. 6. Burg, M.B., and J. D. Ferraris. 2008. Intracellular organic osmolytes: function and regulation. J. Biol. Chem. 283: 7309- 7313. 7. Dennis, J. J., and G. J. Zylstra. 1998. Plasposons: modular self-cloning mini-transposon derivatives for rapid genetic analysis of gram-negative bacterial genomes. Appl. Environ. Microbiol. 64:2710-2715. 8. Goodwin, K. D., R. K. Varner, P. M. Crill, and R. S. Oremland. 2001. Consumption of tropospheric levels of methyl bromide by C1 compound-utilizing bacteria and comparison to saturation kinetics. Appl. Environ. Microbiol. 67: 5437-5443. 9. Jones, J. G. and E. Bellion. 1991. In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA. J. Biol. Chem. 266: 11705-11713. 10. Kanvinde, L., and G. R. K. Sastry. 1990. Agrobacterium tumefaciens is a diazotrophic bacterium. Appl. Environ. Microbiol. 56: 2087-2092. 11. Latypova, E., S. Yang, Y-S. Wang, T. Wang, T. A. Chavkin, M. Hackett, H. 177 Schäfer, and M. G. Kalyuzhnaya. 2010. Genetics of the glutamate-mediated methylamine utilization pathway in the facultative methylotrophic beta-proteobacterium Methyloversatilis uniersalis FAM5. Mol. Microbiol. 75: 426-439. 12. Pollock, R.J., and L. B. Hersh. 1971. N-methylglutamate synthetase: purification and properties of the enzyme. J. Biol. Chem. 246: 4737-4743. 13. Schäfer, A., A. Tauch, W. Jager, J. Kalinowshi, G. Thierbach, and A. Pühler. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73.
URI: http://wrap.warwick.ac.uk/id/eprint/5795

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