The Library
Rapid virulence annotation (RVA) : identification of virulence factors using a bacterial genome library and multiple invertebrate hosts
Tools
Tools
Tools
Waterfield, Nicholas R., Sanchez-Contreras, M., Eleftherianos, I., Dowling, A., Yang, G., Wilkinson, P., Parkhill, J., Thomson, N., Reynolds, S. E., Bode, Helge B., Dorus, S. and ffrench-Constant, R. H. (2008) Rapid virulence annotation (RVA) : identification of virulence factors using a bacterial genome library and multiple invertebrate hosts. Proceedings of the National Academy of Sciences of the United States of America, Volume 105 (Number 41). pp. 15967-15972. doi:10.1073/pnas.0711114105
Research output not available from this repository, contact author.
Official URL: http://dx.doi.org/10.1073/pnas.0711114105
Abstract
Current sequence databases now contain numerous whole genome sequences of pathogenic bacteria. However, many of the predicted genes lack any functional annotation. We describe an assumption-free approach, Rapid Virulence Annotation (RVA), for the high-throughput parallel screening of genomic libraries against four different taxa: insects, nematodes, amoeba, and mammalian macrophages. These hosts represent different aspects of both the vertebrate and invertebrate immune system. Here, we apply RVA to the emerging human pathogen Photorhabdus asymbiotica using “gain of toxicity” assays of recombinant Escherichia coli clones. We describe a wealth of potential virulence loci and attribute biological function to several putative genomic islands, which may then be further characterized using conventional molecular techniques. The application of RVA to other pathogen genomes promises to ascribe biological function to otherwise uncharacterized virulence genes.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Medicine > Warwick Medical School |
||||
| Journal or Publication Title: | Proceedings of the National Academy of Sciences of the United States of America | ||||
| Publisher: | National Academy of Sciences | ||||
| ISSN: | 0027-8424 | ||||
| Official Date: | 2008 | ||||
| Dates: |
|
||||
| Volume: | Volume 105 | ||||
| Number: | Number 41 | ||||
| Page Range: | pp. 15967-15972 | ||||
| DOI: | 10.1073/pnas.0711114105 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Related URLs: |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
