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Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis
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Pereira Morais, Marta P., Marshall, Dominic, Flower, Stephen E., Caunt, Christopher J., James, Tony D., Williams, Robert J., Waterfield, Nicholas R. and van den Elsen, Jean M. H. (2013) Analysis of protein glycation using fluorescent phenylboronate gel electrophoresis. Scientific Reports, Volume 3 . Article number 1437 . doi:10.1038/srep01437 ISSN 2045-2322.
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Official URL: http://dx.doi.org/10.1038/srep01437
Abstract
Glycated proteins are important biomarkers for age-related disorders, however their analysis is challenging because of the complexity of the protein-carbohydrate adducts. Here we report a method that enables the detection and identification of individual glycated proteins in complex samples using fluorescent boronic acids in gel electrophoresis. Using this method we identified glycated proteins in human serum, insect hemolymph and mouse brain homogenates, confirming this technique as a powerful proteomics tool that can be used for the identification of potential disease biomarkers.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Scientific Reports | ||||
Publisher: | Nature Publishing Group | ||||
ISSN: | 2045-2322 | ||||
Official Date: | 2013 | ||||
Dates: |
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Volume: | Volume 3 | ||||
Page Range: | Article number 1437 | ||||
DOI: | 10.1038/srep01437 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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