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Identification by mass spectrometry of CMP-NANA in diffusible material released from high Mr blood cell fractions that confers serum resistance on gonococci
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Parsons, Nicholas R., Ashton, Peter R., Constantinidou, Chrystala, Cole, Jeffrey A. and Smith, Harry (1993) Identification by mass spectrometry of CMP-NANA in diffusible material released from high Mr blood cell fractions that confers serum resistance on gonococci. Microbial Pathogenesis, Volume 14 (Number 4). pp. 329-335. doi:10.1006/mpat.1993.1032 ISSN 0882-4010.
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Official URL: http://dx.doi.org/10.1006/mpat.1993.1032
Abstract
In previous work, a low M(r) component from human blood which converts serum-sensitive gonococci to resistance was shown to be indistinguishable from cytidine 5'-monophospho-N-acetyl neuraminic acid (CMP-NANA) by seven criteria. However, the presence of CMP-NANA was not proved by physicochemical methods. Purified, high M(r) fractions from human blood cells, which confer serum resistance on gonococci and enhance the transfer of sialyl groups from CMP-NANA to lipopolysaccharide (LPS) by a sialyltransferase in gonococcal extracts, were rechromatographed on DEAE Sepharose CL-6B. Both activities co-eluted from the column but on dialysis were found in the diffusate. After desalting the diffusate with Sephadex G10, the presence of CMP-NANA was proved by mass spectrometry. This confirmed previous work and is the first unequivocal demonstration of CMP-NANA in constituents of human blood cells.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Microbial Pathogenesis | ||||
Publisher: | Academic Press | ||||
ISSN: | 0882-4010 | ||||
Official Date: | April 1993 | ||||
Dates: |
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Volume: | Volume 14 | ||||
Number: | Number 4 | ||||
Page Range: | pp. 329-335 | ||||
DOI: | 10.1006/mpat.1993.1032 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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