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Comparative genomic hybridization detects secondary chromosomal deletions in escherichia coli K-12 MG1655 mutants and highlights instability in the flhDC region
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Hobman, J. L., Patel, M. D., Hidalgo-Arroyo, G. A., Cariss, S. J. L., Avison, M. B., Penn, C. W. and Constantinidou, Chrystala (2007) Comparative genomic hybridization detects secondary chromosomal deletions in escherichia coli K-12 MG1655 mutants and highlights instability in the flhDC region. Journal of Bacteriology, Volume 189 (Number 24). pp. 8786-8792. doi:10.1128/JB.00977-07 ISSN 0021-9193.
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Official URL: http://dx.doi.org/10.1128/JB.00977-07
Abstract
The use of whole-genome microarrays for monitoring mutagenized or otherwise engineered genetic derivatives is a potentially powerful tool for checking genomic integrity. Using comparative genomic hybridization of a number of unrelated, directed deletion mutants in Escherichia coli K-12 MG1655, we identified unintended secondary genomic deletions in the flhDC region in Δfnr, Δcrp, and ΔcreB mutants. These deletions were confirmed by PCR and phenotypic tests. Our findings show that nonmotile progeny are found in some MG1655 directed deletion mutants, and studies on the effects of gene knockouts should be viewed with caution when the mutants have not been screened for the presence of secondary deletions or confirmed by other methods.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Journal of Bacteriology | ||||
Publisher: | American Society for Microbiology | ||||
ISSN: | 0021-9193 | ||||
Official Date: | December 2007 | ||||
Dates: |
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Volume: | Volume 189 | ||||
Number: | Number 24 | ||||
Page Range: | pp. 8786-8792 | ||||
DOI: | 10.1128/JB.00977-07 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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