The Library
Deletion of a previously uncharacterized flagellar-hook-length control gene fliK modulates the 54-dependent regulon in Campylobacter jejuni
Tools
Kamal, N., Dorrell, N., Jagannathan, A., Turner, S. M., Constantinidou, Chrystala, Studholme, David J., Marsden, G., Hinds, J., Laing, K. G., Wren, B. W. and Penn, C. W. (2007) Deletion of a previously uncharacterized flagellar-hook-length control gene fliK modulates the 54-dependent regulon in Campylobacter jejuni. Microbiology, Volume 153 (Number 9). pp. 3099-3111. doi:10.1099/mic.0.2007/007401-0 ISSN 1350-0872.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1099/mic.0.2007/007401-0
Abstract
A previously unannotated, putative fliK gene was identified in the Campylobacter jejuni genome based on sequence analysis; deletion mutants in this gene had a ‘polyhook’ phenotype characteristic of fliK mutants in other genera. The mutants greatly overexpressed the σ54-dependent flagellar hook protein FlgE, to form unusual filamentous structures resembling straight flagella in addition to polyhooks. The genome sequence reveals only one gene predicted to encode an orthologue of the NtrC-family activator required for σ54-dependent transcription. Hence, all σ54-dependent genes in the genome would be overexpressed in the fliK mutant together with flgE. Microarray analysis of genome-wide transcription in the mutant showed increased transcription of a subset of genes, often downstream of σ54-dependent promoters identified by a quality-predictive algorithm applied to the whole genome. Assessment of genome-wide transcription in deletion mutants in rpoN, encoding σ54, and in the σ54-activator gene flgR, showed reciprocally reduced transcription of genes that were overexpressed in the fliK mutant. The fliA (σ28)-dependent regulon was also analysed. Together the data clearly define the roles of the alternative sigma factors RpoN and FliA in flagellar biogenesis in C. jejuni, and identify additional putative members of their respective regulons.
Item Type: | Journal Article | ||||
---|---|---|---|---|---|
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
Journal or Publication Title: | Microbiology | ||||
Publisher: | Society for General Microbiology | ||||
ISSN: | 1350-0872 | ||||
Official Date: | September 2007 | ||||
Dates: |
|
||||
Volume: | Volume 153 | ||||
Number: | Number 9 | ||||
Page Range: | pp. 3099-3111 | ||||
DOI: | 10.1099/mic.0.2007/007401-0 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
View Item |