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Global responses of Escherichia colito adverse conditions determined by microarrays and FT-IR spectroscopy
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Moen, Birgitte, Janbu, Astrid Oust, Langsrud, Solveig, Langsrud, Øyvind, Hobman, Jon L., Constantinidou, Chrystala, Kohler, Achim and Rudi, Knut (2009) Global responses of Escherichia colito adverse conditions determined by microarrays and FT-IR spectroscopy. Canadian Journal of Microbiology, Volume 55 (Number 6). pp. 714-728. doi:10.1139/W09-016 ISSN 0008-4166.
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Official URL: http://dx.doi.org/10.1139/W09-016
Abstract
The global gene expression and biomolecular composition in an Escherichia coli model strain exposed to 10 adverse conditions (sodium chloride, ethanol, glycerol, hydrochloric and acetic acid, sodium hydroxide, heat (46 °C), and cold (15 °C), as well as ethidium bromide and the disinfectant benzalkonium chloride) were determined using DNA microarrays and Fourier transform infrared (FT-IR) spectroscopy. In total, approximately 40% of all investigated genes (1682/4279 genes) significantly changed expression, compared with a nonstressed control. There were, however, only 3 genes (ygaW (unknown function), rmf (encoding a ribosomal modification factor), and ghrA (encoding a glyoxylate/hydroxypyruvate reductase)) that significantly changed expression under all conditions (not including benzalkonium chloride). The FT-IR analysis showed an increase in unsaturated fatty acids during ethanol and cold exposure, and a decrease during acid and heat exposure. Cold conditions induced changes in the carbohydrate composition of the cell, possibly related to the upregulation of outer membrane genes (glgAP and rcsA). Although some covariance was observed between the 2 data sets, principle component analysis and regression analyses revealed that the gene expression and the biomolecular responses are not well correlated in stressed populations of E. coli, underlining the importance of multiple strategies to begin to understand the effect on the whole cell.
Item Type: | Journal Article | ||||
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Journal or Publication Title: | Canadian Journal of Microbiology | ||||
Publisher: | N R C Research Press | ||||
ISSN: | 0008-4166 | ||||
Official Date: | June 2009 | ||||
Dates: |
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Volume: | Volume 55 | ||||
Number: | Number 6 | ||||
Page Range: | pp. 714-728 | ||||
DOI: | 10.1139/W09-016 | ||||
Status: | Peer Reviewed | ||||
Publication Status: | Published | ||||
Access rights to Published version: | Restricted or Subscription Access |
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