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Genome analysis of a highly virulent serotype 1 strain of streptococcus pneumoniae from West Africa
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Williams, Tiffany M., Loman, Nicholas J., Ebruke, Chinelo, Musher, Daniel M., Adegbola, Richard A., Pallen, Mark J., Weinstock, George M. and Antonio, Martin (2012) Genome analysis of a highly virulent serotype 1 strain of streptococcus pneumoniae from West Africa. PLoS One, Volume 7 (Number 10). Article number e26742. doi:10.1371/journal.pone.0026742 ISSN 1932-6203.
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Official URL: http://dx.doi.org/10.1371/journal.pone.0026742
Abstract
Streptococcus pneumoniae is a leading cause of pneumonia, meningitis, and bacteremia, estimated to cause 2 million deaths annually. The majority of pneumococcal mortality occurs in developing countries, with serotype 1 a leading cause in these areas. To begin to better understand the larger impact that serotype 1 strains have in developing countries, we characterized virulence and genetic content of PNI0373, a serotype 1 strain from a diseased patient in The Gambia. PNI0373 and another African serotype 1 strain showed high virulence in a mouse intraperitoneal challenge model, with 20% survival at a dose of 1 cfu. The PNI0373 genome sequence was similar in structure to other pneumococci, with the exception of a 100 kb inversion. PNI0373 showed only15 lineage specific CDS when compared to the pan-genome of pneumococcus. However analysis of non-core orthologs of pneumococcal genomes, showed serotype 1 strains to be closely related. Three regions were found to be serotype 1 associated and likely products of horizontal gene transfer. A detailed inventory of known virulence factors showed that some functions associated with colonization were absent, consistent with the observation that carriage of this highly virulent serotype is unusual. The African serotype 1 strains thus appear to be closely related to each other and different from other pneumococci despite similar genetic content.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QH Natural history > QH301 Biology Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General) |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Streptococcus pneumoniae -- Gambia, Bacterial genetics, Bacterial genomes, Pneumonia , Meningitis | ||||
| Journal or Publication Title: | PLoS One | ||||
| Publisher: | Public Library of Science | ||||
| ISSN: | 1932-6203 | ||||
| Official Date: | 2012 | ||||
| Dates: |
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| Volume: | Volume 7 | ||||
| Number: | Number 10 | ||||
| Page Range: | Article number e26742 | ||||
| DOI: | 10.1371/journal.pone.0026742 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Open Access (Creative Commons) | ||||
| Date of first compliant deposit: | 26 December 2015 | ||||
| Date of first compliant Open Access: | 26 December 2015 | ||||
| Funder: | National Institutes of Health (U.S.) (NIH), National Human Genome Research Institute (U.S.) (NHGRI), Medical Research Council (Great Britain) (MRC) | ||||
| Grant number: | U54HG003079, U54HG004968 (NIH-NHGRI) |
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