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Is proteomics a reliable tool to probe the oxidative folding of bacterial membrane proteins?
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Goosens, Vivianne J., Mars, Ruben A. T., Akeroyd, Michiel, Vente, Andre, Dreisbach, Annette, Denham, Emma, Kouwen, Thijs R. H. M., van Rij, Tjeerd, Olsthoorn, Maurien and Dijl, Jan Maarten van (2013) Is proteomics a reliable tool to probe the oxidative folding of bacterial membrane proteins? Antioxidants & Redox Signaling, Volume 18 (Number 10). pp. 1159-1164. doi:10.1089/ars.2012.4664 ISSN 1523-0864.
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Official URL: http://dx.doi.org/10.1089/ars.2012.4664
Abstract
The oxidative folding of proteins involves disulfide bond formation, which is usually catalyzed by thiol-disulfide oxidoreductases (TDORs). In bacteria, this process takes place in the cytoplasmic membrane and other extracytoplasmic compartments. While it is relatively easy to study oxidative folding of water-soluble proteins on a proteome-wide scale, this has remained a major challenge for membrane proteins due to their high hydrophobicity. Here, we have assessed whether proteomic techniques can be applied to probe the oxidative folding of membrane proteins using the Gram-positive bacterium Bacillus subtilis as a model organism. Specifically, we investigated the membrane proteome of a B. subtilis bdbCD mutant strain, which lacks the primary TDOR pair BdbC and BdbD, by gel-free mass spectrometry. In total, 18 membrane-associated proteins showed differing behavior in the bdbCD mutant and the parental strain. These included the ProA protein involved in osmoprotection. Consistent with the absence of ProA, the bdbCD mutant was found to be sensitive to osmotic shock. We hypothesize that membrane proteomics is a potentially effective approach to profile oxidative folding of bacterial membrane proteins. Antioxid. Redox Signal. 18, 1159–1164.
| Item Type: | Journal Article | ||||
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Journal or Publication Title: | Antioxidants & Redox Signaling | ||||
| Publisher: | Mary Ann Liebert, Inc. Publishers | ||||
| ISSN: | 1523-0864 | ||||
| Official Date: | 2013 | ||||
| Dates: |
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| Volume: | Volume 18 | ||||
| Number: | Number 10 | ||||
| Page Range: | pp. 1159-1164 | ||||
| DOI: | 10.1089/ars.2012.4664 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
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