The Library
The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168
Tools
Tools
Tools
Kouwen, T. R. H. M., Trip, E. N., Denham, Emma, Sibbald, M. J. J. B., Dubois, J. -Y. F. and van Dijl, J. M. (2009) The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168. Antimicrobial Agents and Chemotherapy, Volume 53 (Number 11). pp. 4702-4711. doi:10.1128/AAC.00439-09 ISSN 0066-4804.
Research output not available from this repository.
Request-a-Copy directly from author or use local Library Get it For Me service.
Official URL: http://dx.doi.org/10.1128/AAC.00439-09
Abstract
Bacillus subtilis strain 168 produces the extremely stable and broad-spectrum lantibiotic sublancin 168. Known sublancin 168-susceptible organisms include important pathogens, such as Staphylococcus aureus. Nevertheless, since its discovery, the mode of action of sublancin 168 has remained elusive. The present studies were, therefore, aimed at the identification of cellular determinants for bacterial susceptibility toward sublancin 168. Growth inhibition and competition assays on plates and in liquid cultures revealed that sublancin 168-mediated growth inhibition of susceptible B. subtilis and S. aureus cells is affected by the NaCl concentration in the growth medium. Added NaCl did not influence the production, activity, or stability of sublancin 168 but, instead, lowered the susceptibility of sensitive cells toward this lantibiotic. Importantly, the susceptibility of B. subtilis and S. aureus cells toward sublancin 168 was shown to depend on the presence of the large mechanosensitive channel of conductance MscL. In contrast, MscL was not involved in susceptibility toward the bacteriocin nisin or Pep5. Taken together, our unprecedented results demonstrate that MscL is a critical and specific determinant in bacterial sublancin 168 susceptibility that may serve either as a direct target for this lantibiotic or as a gate of entry to the cytoplasm.
| Item Type: | Journal Article | ||||
|---|---|---|---|---|---|
| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
||||
| Journal or Publication Title: | Antimicrobial Agents and Chemotherapy | ||||
| Publisher: | American Society for Microbiology | ||||
| ISSN: | 0066-4804 | ||||
| Official Date: | 2009 | ||||
| Dates: |
|
||||
| Volume: | Volume 53 | ||||
| Number: | Number 11 | ||||
| Page Range: | pp. 4702-4711 | ||||
| DOI: | 10.1128/AAC.00439-09 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access |
Request changes or add full text files to a record
Repository staff actions (login required)
 |
View Item |
