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Construction of a novel shuttle vector for use in Haemophilus influenzae and H. parainfluenzae
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Robinson, Esther R., Juhas, Mario, Hood, Derek W. and Crook, Derrick (2010) Construction of a novel shuttle vector for use in Haemophilus influenzae and H. parainfluenzae. Journal of Microbiological Methods, 83 (3). pp. 330-334. doi:10.1016/j.mimet.2010.09.010 ISSN 0167-7012.
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Official URL: http://dx.doi.org/10.1016/j.mimet.2010.09.010
Abstract
Haemophilus influenzae is an important human pathogen. A number of complete genome sequences of various haemophili are available; however, functional studies have been limited by the lack of an effective shuttle vector which functions in all strains. Here, we have constructed a shuttle vector, pEJ6, which transfers genes between Escherichia coli and H. influenzae and H. parainfluenzae. The vector contains an origin of replication from pLS88 which is functional in E. coli and H. influenzae. In addition it contains an RP4 mobilisation region. The vector can be introduced by electroporation and conjugation into capsulate and non-typeable H. influenzae and is functional for allelic replacement and mutant complementation. The vector will be useful for investigating gene function in Haemophilus spp.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QR Microbiology | ||||||||||
Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Haemophilus influenzae, Haemophilus parainfluenzae, Genetic vectors, Complementation (Genetics) | ||||||||||
Journal or Publication Title: | Journal of Microbiological Methods | ||||||||||
Publisher: | Elsevier | ||||||||||
ISSN: | 0167-7012 | ||||||||||
Official Date: | December 2010 | ||||||||||
Dates: |
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Volume: | 83 | ||||||||||
Number: | 3 | ||||||||||
Number of Pages: | 5 | ||||||||||
Page Range: | pp. 330-334 | ||||||||||
DOI: | 10.1016/j.mimet.2010.09.010 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Open Access (Creative Commons) | ||||||||||
Funder: | Wellcome Trust (London, England), Oxford Biomedical Research Centre | ||||||||||
Grant number: | WT077183MF (WT) |
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