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Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056
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Juhas, Mario, Dimopoulou, Ioanna, Robinson, Esther R., Elamin, Abdel, Harding, Rosalind, Hood, Derek W. and Crook, Derrick (2013) Identification of another module involved in the horizontal transfer of the Haemophilus genomic island ICEHin1056. Plasmid, 70 (2). pp. 277-283. doi:10.1016/j.plasmid.2013.05.008 ISSN 0147-619X.
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Official URL: http://dx.doi.org/10.1016/j.plasmid.2013.05.008
Abstract
A significant part of horizontal gene transfer is facilitated by genomic islands. Haemophilus influenzae genomic island ICEHin1056 is an archetype of a genomic island that accounts for pandemic spread of antibiotics resistance. ICEHin1056 has modular structure and harbors modules involved in type IV secretion and integration. Previous studies have shown that ICEHin1056 encodes a functional type IV secretion system; however, other modules have not been characterized yet. Here we show that the module on the 5′ extremity of ICEHin1056 consists of 15 genes that are well conserved in a number of related genomic islands. Furthermore by disrupting six genes of the investigated module of ICEHin1056 by site-specific mutagenesis we demonstrate that in addition to type IV secretion system module, the investigated module is also important for the successful conjugal transfer of ICEHin1056 from donor to recipient cells.
Item Type: | Journal Article | ||||||||||
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Subjects: | Q Science > QH Natural history > QH426 Genetics Q Science > QR Microbiology |
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Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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Library of Congress Subject Headings (LCSH): | Haemophilus influenzae -- Genetic aspects, Genetic transformation, Genomics | ||||||||||
Journal or Publication Title: | Plasmid | ||||||||||
Publisher: | Academic Press | ||||||||||
ISSN: | 0147-619X | ||||||||||
Official Date: | September 2013 | ||||||||||
Dates: |
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Volume: | 70 | ||||||||||
Number: | 2 | ||||||||||
Page Range: | pp. 277-283 | ||||||||||
DOI: | 10.1016/j.plasmid.2013.05.008 | ||||||||||
Status: | Peer Reviewed | ||||||||||
Publication Status: | Published | ||||||||||
Access rights to Published version: | Restricted or Subscription Access | ||||||||||
Funder: | Medical Research Council (Great Britain) (MRC) | ||||||||||
Adapted As: |
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