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Increased sulfate uptake by E. coli overexpressing the SLC26-related SulP protein Rv1739c from Mycobacterium tuberculosis
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Zolotarev, Alexander S., Unnikrishnan, Meera, Shmukler, Boris E., Clark, J. S. (Jeffrey S.), Vandorpe, David H., Grigorieff, Nikolaus, Rubin, Eric J. and Alper, Seth L. (2008) Increased sulfate uptake by E. coli overexpressing the SLC26-related SulP protein Rv1739c from Mycobacterium tuberculosis. Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology, Volume 149 (Number 3). pp. 255-266. doi:10.1016/j.cbpa.2007.12.005 ISSN 1095-6433.
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Official URL: http://dx.doi.org/10.1016/j.cbpa.2007.12.005
Abstract
Growth and virulence of mycobacteria requires sulfur uptake. The Mycobacterium tuberculosis genome contains, in addition to the ABC sulfate permease cysTWA, three SLC26-related SulP genes of unknown function. We report that induction of Rv1739c expression in E. coli increased bacterial uptake of sulfate, but not Cl−, formate, or oxalate. Uptake was time-dependent, maximal at pH 6.0, and exhibited a K1/2 for sulfate of 4.0 μM. Na+-independent sulfate uptake was not reduced by bicarbonate, nitrate, or phosphate, but was inhibited by sulfite, selenate, thiosulfate, N-ethylmaleimide and carbonyl cyanide 3-chloro-phenylhydrazone. Sulfate uptake was also increased by overexpression of the Rv1739c transmembrane domain, but not of the cytoplasmic C-terminal STAS domain. Mutation to serine of the three cysteine residues of Rv1739c did not affect magnitude, pH-dependence, or pharmacology of sulfate uptake. Expression of Rv1739c in a M. bovis BCG strain lacking the ABC sulfate permease subunit CysA could not complement sulfate auxotrophy. Moreover, inducible expression of Rv1739c in an E. coli strain lacking CysA did not increase sulfate uptake by intact cells. Our data show that facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, and suggest that Rv1739c may be a CysTWA-dependent sulfate transporter.
| Item Type: | Journal Article | ||||
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| Subjects: | Q Science > QP Physiology Q Science > QR Microbiology |
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| Divisions: | Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School > Biomedical Sciences > Microbiology & Infection Faculty of Science, Engineering and Medicine > Medicine > Warwick Medical School |
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| Library of Congress Subject Headings (LCSH): | Mycobacterium tuberculosis, Escherichia coli, Sulfur -- Physiological effect, Sulfates -- Physiological transport, Mycobacterium bovis | ||||
| Journal or Publication Title: | Comparative Biochemistry and Physiology Part A : Molecular & Integrative Physiology | ||||
| Publisher: | Elsevier | ||||
| ISSN: | 1095-6433 | ||||
| Official Date: | March 2008 | ||||
| Dates: |
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| Volume: | Volume 149 | ||||
| Number: | Number 3 | ||||
| Page Range: | pp. 255-266 | ||||
| DOI: | 10.1016/j.cbpa.2007.12.005 | ||||
| Status: | Peer Reviewed | ||||
| Publication Status: | Published | ||||
| Access rights to Published version: | Restricted or Subscription Access | ||||
| Funder: | National Institutes of Health (U.S.) (NIH) | ||||
| Grant number: | R01 DK43495, DK34854, T32 DK07199 (NIH) |
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